ANTIMICROBIAL ACTIVITY OF HORSE CHESTNUT (AESCULUS HIPPOCASTANUM) AGAINST CYSTIC FIBROSIS PATHOGENIC STRAINS


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Abstract

Introduction. Cystic fibrosis is a common inherited disease that affects many organs and often leads to death. The treatment complexity is the resistance of its pathogenic strains to currently available drugs. The problem can be solved by the use of antibacterial herbal drugs. One of the sources of biologically active substances for antimicrobial herbal remedies can be common horse chestnut (Aesculus hippocastanum). Objective: to conduct a screening study of the antibacterial activity of aqueous alcohol extracts from common horse chestnut flowers and buds, as well as chloroform extract and fraction of an individual substance of flavonoid nature, which are obtained from the plant buds. Material and methods. The investigation objects were aqueous alcohol extracts from horse chestnut buds and flowers in 40, 70 and 96% ethanol, as well as alcohol solutions of chloroform extracts of the flavonoid fraction from the plant buds. The minimum inhibitory concentration (MIC) was estimated by the double serial broth dilution method. The clinical strains Burkholderia cenocepacia ST 208, Burkholderia multivorans, and Pseudomonas aeruginosa from patients with cystic fibrosis were used as test cultures. Results. The extracts from horse chestnut flowers were found to have a broad-spectrum antimicrobial activity and a pronounced bactericidal effect. The chloroform extract from the buds and the flavonoid fraction had lower activity. The extracts from horse chestnut buds showed a narrow-spectrum activity with sustained activity as antibacterial agents against Burkholderia cenocepacia ST 208 and Pseudomonas aeruginosa. Conclusion. The results of the investigation can be used as a rationale for including novel types of medicinal plant materials, as well as their based preparations in the State Pharmacopoeia of the Russian Federation.

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About the authors

Pavel Viktorovich Belov

Samara State Medical University

Email: almelion@rambler.ru
postgraduate Department of pharmacognosy with botany and the basics of phytotherapy 89, Chapaevskaya St., Samara 443099, Russian Federation

Vladimir Alexandrovich Kurkin

Samara State Medical University

Email: kurkinvladimir@yandex.ru
doctor of Pharmaceutical Sciences, Professor, Head of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy 89, Chapaevskaya St., Samara 443099, Russian Federation

Vitaly Mikhailovich Ryzhov

Samara State Medical University

Email: lavr_rvm@mail.ru
PhD of Pharmaceutical Sciences, Associate Professor of Pharmacognosy with Botany and the Basics of Phytotherapy 89, Chapaevskaya St., Samara 443099, Russian Federation

Artem Viktorovich Lyamin

Samara State Medical University

Email: avlyamin@rambler.ru
сandidate of Medical Science, Associate Professor Department of General and Clinical Microbiology, Immunology and Allergology 89, Chapaevskaya St., Samara 443099, Russian Federation

Olga Vladimirovna Kondratenko

Samara State Medical University

Email: microbiology@samsmu.ru
сandidate of Medical Science, Associate ProfessorDepartment of General and Clinical Microbiology, Immunology and Allergology 89, Chapaevskaya St., Samara 443099, Russian Federation

Alexander Vladimirovich Pomogaibin

Academician S.P. Korolev Samara National Research University

Email: sambg@ssau.ru
сandidate of Biological Science, Deputy Director of the Botanical garden 34, Moskovskoe Shosse, Samara 443086, Russian Federation

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