Quantitative determination of the mangiferine in Hedysarum caucasicum M.Bieb.by HPLC method

Introduction. The species of the genus Hedysarum L., Fabaceae family are of considerable interest from a pharmacognostic point of view. In these species, one of the main biologically active substances is xanthones, xanthone glycoside mangiferin, having pronounced antibacterial and antiviral activity, with respect to DNA-containing viruses. Due to these pharmacological properties, the search for additional raw sources of mangiferin is promising. Objective: development of a methodology for quantitative determination of mangiferin in the aerial part of Hedysarum caucasicum M. Bieb. by HPLC. Material and methods. The objects of the study were samples of the aerial part of Hedysarum caucasicum M. Bieb. collected on the territory of Kabardino-Balkary and Karachai-Cherkessya republics. Electronic spectra were recorded on Shimadzu Prominence LC-20AD liquid chromatograph, with a DGU-20A3R degasser. Mangiferin Sigma-Aldrich was used as a standard sample. The mobile phase was a mixture of buffer solution, acetonitrile and methanol in a ratio of 81:16:3. Results. The technique takes into account the basic physical and chemical properties of xanthones, is characterized by reproducibility, high accuracy, simplicity of execution, allows to carry out both screening evaluation of various raw objects containing mangiferin, as well as standardization of prepared medicinal plant raw materials. As a result, 0.13-0.15% of mangiferin was found in the aerial organs of Hedysarum caucasicum. Conclusion. The results show that there is sufficient mangiferin in the aerian part of the Hedysarum caucasicum, which makes it possible to recommend mangiferin as an additional raw material source as an antiviral agent of domestic origin.