Evaluation of the quality and biological activity of the «BioChaga» birch fungus extract

Introduction. «BioChaga» is a natural extract of the birch mushroom chaga, produced according to the author's technology of the Irkutsk company SibPribor LLC under the trademark «Baikal Legend ». The high quality of «BioChaga» is due to the combination of environmentally friendly chaga medicinal plant raw materials rich in biologically active substances of the Baikal region and th e unique author's technology of its processing. The result is a fast-soluble «BioChaga» powder with low density, high biological activity and bioavailability. The purpose of the study: a comprehensive study of the composition and content of BAS, biological activity of the «BioChaga » extract in connection with the peculiarities of its production technology. Material and methods. To determine the physicochemical properties of «BioChaga» extract we used the following methods: extraction / gravimetry, spectrophotometry, the qualitative composition was studied using high-performance liquid chromatography. To determine the biological activity of «BioChaga », the antiradical activity of the extract to organic and inorganic radicals of natural and artificial origin was determined by spectrometry and coulometry. The antioxidant potential was evaluated using spectrophotometry and fluorimetry to determine the total antioxidant potential and the ability to absorb oxygen radicals. The iron-reducing and iron- chelating potential of «BioChaga» was determined by spectrophotometry. Results. The physico-chemical characteristics of the «BioChaga» composition had a high level of compounds characteristic of the chaga: low molecular weight phenolic compounds - 57,00±1,48 mg/kg, high molecular weight phenolic compounds (melanin complex) - 24,9±1,2%. The nature of the distribution of fragments of the melanin complex shows a predominant content of o-dioxybenzoyl fragments, which suggests the presence of high biological activity in the «BioChaga». It has been shown that the «BioChaga» has antioxidant, antiradical and iron-reducing activity. In addition, the extract has proven to be a good inactivator of nitric oxide molecules, which indicates its anti-inflammatory properties. Conclusion. «BioChaga» is a highly active natural complex agent characterized as an effective antioxidant created using a unique author's technology. Its high biological activity indicates the effectiveness of the extract and allows it to be recommended as a means of prevention and protection against oxidative stress