Approaches to grass standardization the moldovan snakehead

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Abstract

Introduction. Dracocephalum moldavica L. is an annual herbaceous plant of the Lamiaceae family. The above-ground part of this plant contains various biologically active substances – essential oil, flavonoids and their glycosides, phenolic acids, triterpenoids, steroids, lignans, anthocyanins, iridoids, polysaccharides, pectin substances. A method for obtaining a pharmaceutical substance in the form of a dry extract has been developed at VILAR. In vivo and in vitro experiments have shown that it has pronounced anti-inflammatory and gastroprotective activities, which may be promising for its further study. One of the main conditions for ensuring the quality of the developed substance is the solution of issues of standardization of the herb of the Moldavian dragonhead and the extract obtained from it.

Objective: To establish criteria of authenticity and quality indicators that allow for quality control of the herb of the Moldavian dragonhead.

Material and methods. The object of the study is dried Moldavian dragonhead herb, harvested in the North Caucasian branch of VILAR during the budding and flowering phases in 2018, in the Middle Volga branch of VILAR and in the VILAR experimental field during the flowering phase in 2020. The entire study was carried out in accordance with the general pharmacopoeial articles of the State Pharmacopoeia of the Russian Federation, XV edition.

Results. As a result of the studies, morphological and anatomical diagnostic features of the raw materials were established, a method for assessing authenticity by TLC with a standard sample of rosmarinic acid was developed, the nomenclature of numerical indicators and their norms were determined. For the quantitative assessment of biologically active substances, a method for determining the amount of phenolic compounds in terms of rosmarinic acid was developed and validated. Analysis of samples of experimental batches of Moldavian dragonhead herb made it possible to establish the standard content of phenolic compounds in terms of rosmarinic acid of at least 4%.

Conclusion. The obtained data allowed us to develop and formalize a normative document – a draft pharmacopoeial article “Moldavian dragonhead herb”.

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About the authors

Elena Anatolyevna Konyaeva

All-Russian Research Institute of Medicinal and Aromatic Plants

Email: lencon64@mail.ru
ORCID iD: 0000-0002-2154-3755
SPIN-code: 8803-2418

Senior Researcher of the Department of Chemistry of Natural Compounds

Russian Federation, Moscow

Olesya Yuryevna Kulyak

All-Russian Research Institute of Medicinal and Aromatic Plants

Email: kulyak-olesya@mail.ru
ORCID iD: 0000-0003-0385-3474
SPIN-code: 9697-1220

Leading Researcher of the Department of Chemistry of Natural Compounds

Russian Federation, Moscow

Andrey Ivanovich Radimich

All-Russian Research Institute of Medicinal and Aromatic Plants

Email: vilarnii.radimich@mail.ru
ORCID iD: 0000-0002-1139-8902
SPIN-code: 5452-2675

Senior Researcher of the Department of Chemistry of Natural Compounds

Russian Federation, Moscow

Alla Evgenievna Burova

All-Russian Research Institute of Medicinal and Aromatic Plants

Email: alla.burova@inbox.ru
ORCID iD: 0000-0003-3883-576X
SPIN-code: 5887-5933

Senior Researcher of the Department of Chemistry of Natural Compounds

Russian Federation, Moscow

Olga Petrovna Sheychenko

All-Russian Research Institute of Medicinal and Aromatic Plants

Email: vilarnii.radimich@mail.ru
ORCID iD: 0000-0001-7099-4192
SPIN-code: 2527-6951

Leading Researcher of the Department of Chemistry of Natural Compounds

Russian Federation, Moscow

Olga Leonidovna Saybel

All-Russian Research Institute of Medicinal and Aromatic Plants

Author for correspondence.
Email: olster@mail.ru
ORCID iD: 0000-0001-8059-5064
SPIN-code: 2842-4587

Head of the Center for Chemistry and Pharmaceutical Technology

Russian Federation, Moscow

References

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  2. Государственный реестр селекционных достижений, допущенных к использованию: Т.1: сорта растений. М.: ФГБНУ «Росинформагротех», 2019; 516. Сайт. URL http://gossortrf.ru (дата обращения 22.04.2024). Яз.рус. Режим доступа: свободный. Текст: электронный. [State Register of Breeding Achievements Approved for Use: Vol. 1: Plant Varieties. Moscow: Federal State Budgetary Scientific Institution Rosinformagrotech, 2019; 516. Website. URL http://gossortrf.ru (accessed 22.04.2024). Language: Russian. Access mode: free. Text: electronic (in Russian)].
  3. Курманова Е.Н., Ферубко Е.В., Шейченко О.П. Оценка противовоспалительных и гастропротективных свойств экстракта змееголовника молдавского. Вопросы биологической, медицинской и фармацевтической химии. 2019; 22 (6): 52–5. [Kurmanova E.N., Ferubko E.V., Sheychenko O.P. Evaluation of anti-inflammatory and gastroprotective properties of Moldavian dragonhead extract. Issues of biological, medical and pharmaceutical chemistry. 2019; 22 (6): 52–5 (in Russian)].
  4. Курманова Е.Н., Ферубко Е.В., Стрелкова Л.Б., Курманов Р.К., Шейченко О.П. Изучение противовоспалительной активности экстракта змееголовника молдавского. Патологическая физиология и экспериментальная терапия. 2020; 64 (1): 108–12. [Kurmanova E.N., Ferubko E.V., Strelkova L.B., Kurmanov R.K., Sheychenko O.P. Study of anti-inflammatory activity of Moldavian dragonhead extract. Pathological physiology and experimental therapy. 2020; 64 (1): 108–12 (in Russian)].
  5. Государственная фармакопея Российской Федерации XV издания [Электронный ресурс]. Министерство здравоохранения Российской Федерации. 2023. Режим доступа: Издания Государственной фармакопеи Российской Федерации (regmed.ru). [The State Pharmacopoeia of The Russian Federation, XIV ed. [Electronic edition]. Ministry of Health of the Russian Federation. 2023. Access mode: Publications of the State Pharmacopoeia of the Russian Federation (regmed.ru) (in Russian)].

Supplementary files

Supplementary Files
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1. JATS XML
2. Fig. 1. Diagnostic features of the anatomical structure of the herb Dragonhead moldavian (1–9, 12–17 – ×400; 10, 11 – ×200)

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3. Fig. 2. TLC chromatogram of the aqueous-alcoholic extract from the herb Moldavian dragonhead: 1 – rosmarinic acid CO; 2 – alcoholic extract of the herb Moldavian dragonhead, North Caucasus branch; 3 – alcoholic extract of the herb Moldavian dragonhead, Middle Volga branch; 4 – alcoholic extract of the herb Moldavian dragonhead, experimental field

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4. Fig. 3. Absorption spectrum of 50% alcohol extract from Moldavian dragonhead herb (1) and rosmarinic acid CO (2)

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