Substantiation of technology and standardization of extraction preparations based on Melissa officinalis herb

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Abstract

Introduction. Melissa officinalis L. is one of the most popular plants due to its rich composition of biologically active compounds. The uniqueness of Melissa officinalis lies in the combination of anxiolytic, sedative, antiviral and antihistamine properties. Currently, the problem of creating import-substituting medicines is very urgent, which should be accompanied by standardization of quantitative analysis methods. In this regard, high-performance liquid chromatography is a promising method. In accordance with the Russian Federation State Budget of the XIV edition, the standardization of Melissa officinalis herb is carried out by spectrophotometry of the content of phenolic compounds (phenylpropanoids) calculated on rosmarinic acid.

The aim. Study to substantiate the technology and methods of standardization of extraction preparations based on the herb of Melissa officinalis.

Material and methods. Melissa officinalis herb cultivated near the village of Alekseevka, Samara region, was used as an object of research (harvest time – July 2024). Tinctures were obtained by modified maceration from Melissa officinalis herb based on an extractant – ethyl alcohol at concentrations of 40% and 70%, from which thick extracts were further developed. These extraction preparations, as well as the resulting infusion (1:10), were analyzed by spectrophotometry and high-performance liquid chromatography.

Results. Using the spectrophotometry method, it was determined that the content of the total phenylpropanoids (calculated on rosmarinic acid) in samples of tincture on 40% ethyl alcohol, tincture on 70% ethyl alcohol; thick extract on 40% ethyl alcohol, thick extract on 70% ethyl alcohol and infusion is 0,83 ± 0,02; 0,65 ± 0,01; 9,07 ± 0,45; 5,58 ± 0,26 and 0,22 ± 0,01% accordingly.

As a result of the conducted research, a method has been developed for the quantitative determination of rosmarinic acid in extraction preparations based on Melissa officinalis herb using the HPLC method. It was determined that the content of rosmarinic acid in the samples of tincture on 40% ethyl alcohol, tincture on 70% ethyl alcohol; thick extract on 40% ethyl alcohol, thick extract on 70% ethyl alcohol and infusion is 0,46 ± 0,01; 0,33 ± 0,01; 4,10 ± 0,20; 5,11 ± 0,25 and 0,14 ± 0,01% accordingly.

Conclusion. A study using spectrophotometry and HPLC revealed shown that 40% ethyl alcohol is the optimof al extractant for extracting the amount of phenylpropanoids and rosmarinic acid from the herb Melissa officinalis. The expediency of standardization of extraction preparations based on Melissa officinalis herbs on content of rosmarinic acid using HPLC method was substantiated.

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About the authors

Alina Irecovna Epifanova

Samara State Medical University

Email: epifanova.a01@mail.ru
ORCID iD: 0009-0004-4481-6981

Postgraduate Student of the Department of Pharmaceutical Technology with a Course in Biotechnology

Russian Federation, Samara

Vladimir Aleksandrovich Kurkin

Samara State Medical University

Email: v.a.kurkin@samsmu.ru
ORCID iD: 0000-0002-7513-9352

Doctor of Pharmaceutical Sciences, Professor, Head of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy

Russian Federation, Samara

Anna Vladimirovna Kurkina

Samara State Medical University

Email: a.v.kurkina@samsmu.ru
ORCID iD: 0000-0002-5028-9186

Doctor of Pharmaceutical Sciences, Professor, Head of the Department of Pharmaceutical Technology with a Course in Biotechnology

Russian Federation, Samara

Artur Rustemovich Mubinov

Samara State Medical University

Email: a.r.mubinov@samsmu.ru
ORCID iD: 0000-0002-4791-4606

Candidate of Pharmaceutical Sciences, Leading Researcher at the Pharmacy Scientific and Educational Center

Russian Federation, Samara

Valery Borisovich Braslavsky

Samara State Medical University

Author for correspondence.
Email: v.b.braslavskii@samsmu.ru
ORCID iD: 0000-0002-6050-3377

Doctor of Pharmaceutical Sciences, Associate Professor at the Department of Pharmacognosy with Botany and the Basics of Phytotherapy

Russian Federation, Samara

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Supplementary files

Supplementary Files
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2. Fig. 1. The structural formula of rosmarinic acid

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3. Fig. 2. Electronic spectra of extraction preparations based on Melissa officinalis herb

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4. Fig. 3. HPLC chromatogram of a standard sample of rosmarinic acid

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5. Fig. 4. HPLC chromatograms of tincture in 40% ethyl alcohol

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6. Fig. 5. HPLC chromatograms of tincture on 70% ethyl alcohol

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7. Fig. 6. Graph of the dependence of the peak height on the concentration of rosmarinic acid in the sample and the equation of linear regression of tincture in 40% ethyl alcohol

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