Eutectic solvents for the extraction of phenolic compounds from the Moldovan Dragonhead

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Abstract

Introduction. Deep eutectic solvents (DES) are of interest to modern pharmaceutical technology as alternative extractants to traditional organic solvents. The prospects for their use are due to low toxicity, biodegradability, the possibility of selective extraction of individual substances and regeneration of the extractant.

The purpose of the study is to develop and optimize a technology for ultrasonic extraction of phenolic acids from the Moldovan dragonhead using DES, and to study the antioxidant activity of the resulting extracts.

Results. 11 DES compositions with different molar ratios of components were obtained; the optimal composition one was selected based on choline and lactic acid in a molar ratio of 1:3. The dependence of the extraction of phenolic compounds on the composition of DES, the influence of water content in the composition of DES on the process of extraction of phenolic compounds have been studied. Data were obtained on the antioxidant activity of the Moldavian snakehead extract obtained using DES.

Conclusion. A method has been developed for the extraction of phenolic acids from the Moldavian snakehead with deep eutectic solvents. The extraction efficiency of the DES-3 mixture based on choline chloride and lactic acid in a molar ratio of 1:3 has been proven. The content of phenolic compounds in terms of rosmarinic acid was 7.72±0.12%, which is 0.23% more than when using 50% ethyl alcohol as an extractant. Using cyclic voltammetry, it was established that the antioxidant activity of extracts obtained using DES is due to the content of rosmarinic acid.

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About the authors

Marina Arkadyevna Dzhavakhyan

Russian University of Medicine; All-Russian Research Institute of Medicinal and Aromatic Plants

Email: akopovamarina13@mail.ru
ORCID iD: 0000-0003-2673-6203

Doctor of Pharmaceutical Sciences, Associate Professor, Deputy Director of the Scientific and Educational Institute for the Development and Introduction of Medicines; Chief Researcher of the Experimental and Technological Department

Russian Federation, Moscow; Moscow

Olesya Konstantinovna Pavelyeva

Russian University of Medicine

Email: pavelieva.olesya@yandex.ru
ORCID iD: 0000-0002-2397-8920

Applicant

Russian Federation, Moscow

Vyacheslav Nikolaevich Dul

Russian University of Medicine; All-Russian Research Institute of Medicinal and Aromatic Plants

Email: dvnslava@yandex.ru
ORCID iD: 0000-0001-7824-4417

Candidate of Pharmaceutical Sciences, Researcher of the Development and Implementation of Innovative Medicines Research Laboratory; Researcher

Russian Federation, Moscow; Moscow

Anna Gurgenovna Kuregyan

Pyatigorsk Medical and Pharmaceutical Institute – a branch of the Volgograd Medical State University

Email: Kooreguan@mail.ru
ORCID iD: 0000-0002-0698-8254

Doctor of Pharmaceutical Sciences, Professor of the Pharmaceutical Chemistry Department

Russian Federation, Pyatigorsk

Tatiana Yurievna Tatarenko-Kozmina

Russian University of Medicine

Email: kosmtina025@gmail.com
ORCID iD: 0009-0000-8569-9209

Doctor of Biological Sciences, Professor, Head of the Medical Biology and Fundamentals of Cellular and Molecular Biotechnology Department

Russian Federation, Moscow

Anatoly Petrovich Pleten

Russian University of Medicine

Author for correspondence.
Email: pleatol@mail.ru
ORCID iD: 0000-0003-4991-2150

Doctor of Biological Sciences, Professor of the Biological Chemistry Department

Russian Federation, Moscow

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Supplementary files

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2. Fig. 1. Content of phenolic compounds depending on the solvent used

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3. Fig. 2. Influence of water content in DES on the extraction process of phenolic compounds

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4. Fig. 3. Cyclic voltammogram of the herb extract of the Moldovan dragonhead based on DES-3

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5. Fig. 4. Cyclic voltammogram of the alcoholic extract of the herb Moldovan snakeheadо

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