Substantiation of technology and standardization of extraction preparations based on Melissa officinalis herb

Introduction. Melissa officinalis L. is one of the most popular plants due to its rich composition of biologically active compounds. The uniqueness of Melissa officinalis lies in the combination of anxiolytic, sedative, antiviral and antihistamine properties. Currently, the problem of creating import-substituting medicines is very urgent, which should be accompanied by standardization of quantitative analysis methods. In this regard, high-performance liquid chromatography is a promising method. In accordance with the Russian Federation State Budget of the XIV edition, the standardization of Melissa officinalis herb is carried out by spectrophotometry of the content of phenolic compounds (phenylpropanoids) calculated on rosmarinic acid.

The aim. Study to substantiate the technology and methods of standardization of extraction preparations based on the herb of Melissa officinalis.

Material and methods. Melissa officinalis herb cultivated near the village of Alekseevka, Samara region, was used as an object of research (harvest time – July 2024). Tinctures were obtained by modified maceration from Melissa officinalis herb based on an extractant – ethyl alcohol at concentrations of 40% and 70%, from which thick extracts were further developed. These extraction preparations, as well as the resulting infusion (1:10), were analyzed by spectrophotometry and high-performance liquid chromatography.

Results. Using the spectrophotometry method, it was determined that the content of the total phenylpropanoids (calculated on rosmarinic acid) in samples of tincture on 40% ethyl alcohol, tincture on 70% ethyl alcohol; thick extract on 40% ethyl alcohol, thick extract on 70% ethyl alcohol and infusion is 0,83 ± 0,02; 0,65 ± 0,01; 9,07 ± 0,45; 5,58 ± 0,26 and 0,22 ± 0,01% accordingly.

As a result of the conducted research, a method has been developed for the quantitative determination of rosmarinic acid in extraction preparations based on Melissa officinalis herb using the HPLC method. It was determined that the content of rosmarinic acid in the samples of tincture on 40% ethyl alcohol, tincture on 70% ethyl alcohol; thick extract on 40% ethyl alcohol, thick extract on 70% ethyl alcohol and infusion is 0,46 ± 0,01; 0,33 ± 0,01; 4,10 ± 0,20; 5,11 ± 0,25 and 0,14 ± 0,01% accordingly.

Conclusion. A study using spectrophotometry and HPLC revealed shown that 40% ethyl alcohol is the optimof al extractant for extracting the amount of phenylpropanoids and rosmarinic acid from the herb Melissa officinalis. The expediency of standardization of extraction preparations based on Melissa officinalis herbs on content of rosmarinic acid using HPLC method was substantiated.