A novel PRE-element from Drosophila virilis genome as a useful model silencer

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Abstract


We tested the activity of the PRE element from the Drosophila virilis genome, which is the homologue of the known Drosophila melanogaster bxdPRE element from the regulatory region of the Ubx gene. It is easy to select unique primers to this element that do not occur in the Drosophila melanogaster genome. We showed that the studied PRE element causes a strong repression of the white marker gene upon insertion into the Drosophila melanogaster genome and interacts with the PcG proteins of the PRC1 and PhoRC complexes.


D. A. Chetverina

Институт биологии гена Российской Академии наук

Author for correspondence.
Email: yermaxbio@yandex.ru

Russian Federation, Москва

A. V. Mikhailova

Институт биологии гена Российской Академии наук

Email: yermaxbio@yandex.ru

Russian Federation, Москва

P. G. Georgiev

Институт биологии гена Российской Академии наук

Email: yermaxbio@yandex.ru

Russian Federation, Москва

M. M. Erokhin

Институт биологии гена Российской Академии наук

Email: yermaxbio@yandex.ru

Russian Federation, Москва

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