Gene dunce localization in the polytene chromosome of Drosophila melanogaster long span batch of adjacent chromosomal structures
- Authors: Khoroshko V.A.1, Pokholkova G.V.1, Zykova T.Y.1, Osadchiy I.S.2, Zhimulev I.F.1,3
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Affiliations:
- Институт молекулярной и клеточной биологии Сибирского отделения Российской Академии наук
- Институт биологии гена Российской Академии наук
- Новосибирский национальный исследовательский государственный университет
- Issue: Vol 484, No 4 (2019)
- Pages: 507-510
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/12572
- DOI: https://doi.org/10.31857/S0869-56524844507-510
- ID: 12572
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Abstract
The molecular and chromosomal localization of the dunce gene was studied. This gene (167.3 kb) consists almost entirely of introns, in which a cluster of seven short tissue-specific genes is located. On the basis of the results of FISH analysis of the gene fragments, we established that the dunce gene is located within nine chromosomal structures (four bands and five interbands), which contradicts the common idea that genes are located in only one structure (band or interband) or at the boundary of these structures. Our results are quite unexpected and original and greatly expand the current understanding of the genetic organization of interphase chromosomes.
About the authors
V. A. Khoroshko
Институт молекулярной и клеточной биологии Сибирского отделения Российской Академии наук
Author for correspondence.
Email: vicerna@mcb.nsc.ru
Russian Federation, Новосибирск
G. V. Pokholkova
Институт молекулярной и клеточной биологии Сибирского отделения Российской Академии наук
Email: vicerna@mcb.nsc.ru
Russian Federation, Новосибирск
T. Yu. Zykova
Институт молекулярной и клеточной биологии Сибирского отделения Российской Академии наук
Email: vicerna@mcb.nsc.ru
Russian Federation, Новосибирск
I. S. Osadchiy
Институт биологии гена Российской Академии наук
Email: vicerna@mcb.nsc.ru
Russian Federation, Москва
I. F. Zhimulev
Институт молекулярной и клеточной биологии Сибирского отделения Российской Академии наук; Новосибирский национальный исследовательский государственный университет
Email: vicerna@mcb.nsc.ru
Academician of the RAS
Russian Federation, НовосибирскReferences
- Zhimulev I.F., Zykova T.Y., Goncharov F.P., Khoroshko V.A., Demakova O.V., Semeshin V.F., Pokholkova G.V., Boldyreva L.V., Demidova D.S., Babenko V.N., Demakov S.A., Belyaeva E.S. Genetic Organization of Interphase Chromosome Bands and Interbands in Drosophila melanogaster // PLoS One. 2014. V. 9. № 7. e101631.
- Belyakin S.N., Christophides G.K., Alekseyenko A.A., Kriventseva E.V,. Belyaeva E.S., Nanayev R.A., Makunin I.V., Kafatos F.C., Zhimulev I.F. Genomic Analysis of Drosophila Chromosome Underreplication Reveals a Link between Replication Control and Transcriptional Territories // Proc. Natl. Acad. Sci. USA. 2005. V. 102. № 23. P. 8269–8274.
- Qiu Y.H., Chen C.N., Malone T., et al. Characterization of the Memory Gene dunce of Drosophila melanogaster // J. Mol. Biol. 1991. V. 222. № 3. P. 553–565.
- Qiu Y., Davis R.L. Genetic Dissection of the Learning / Memory Gene dunce of Drosophila melanogaster // Genes Develop. 1993. V. 7. № 7B. P. 1447–1458.
- Furia M., D’Avino P.P., Crispi S., et al. Dense Cluster of Genes Is Located at the Ecdysone-Regulated 3C Puff of Drosophila melanogaster // J. Mol. Biol. 1993. V. 231. № 2. P. 531–538.
- Семешин В.Ф., Артеро Р., Перес-Алонсо М, Шлома В.В. Электронно-микроскопическая гибридизация in situ проб, меченных dUТР-дигоксигенином, с политенными хромосомами Drosophila melanogaster // Цитология. 1998. Т. 40. № 10. С. 893–894.
- Bridges C.B. Salivary Chromosome Maps: with a Key to the Banding of the Chromosomes of Drosophila melanogaster // J. Hered. 1935. V. 26. № 2. P. 60–64.
- Crick F. General Model for the Chromosomes of Higher Organisms // Nature. 1971. V. 234. № 5323. P. 25–27.
- Paul J. General Theory of Chromosome Structure and Gene Activation in Eukaryotes // Nature. 1972. V. 238. № 5365. P. 444–446.
- Zhimulev I.F. Genetic Organization of Polytene Chromosomes. N.Y.: Acad. Press, 1999. 599 p.