Endothelium-dependent hyperpolarization-mediated relaxation pathway in bovine mesenteric lymph nodes

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Abstract

In vitro, endothelium-dependent relaxation mechanisms of smooth muscle cells of the bovine mesenteric lymph node capsule have been studied. The addition of L-NAME and indomethacin to physiological saline inhibited the production of endothelium NO and prostacyclin. In this solution, tetraethylammonium chloride and TRAM-34 increased the tone of the precontracted lymph nodes. Thus, in bovine mesenteric lymph nodes there is an relaxation mechanism mediated by endothelial hyperpolarization, realized by activating Ca2+-dependent K+-channels of large- and intermediate conductance.

About the authors

G. I. Lobov

Pavlov Institute of Physiology of the Russian Academy of Sciences

Author for correspondence.
Email: gilobov@yandex.ru
Russian Federation, Saint-Petersburg

D. P. Dvoretskii

Pavlov Institute of Physiology of the Russian Academy of Sciences

Email: gilobov@yandex.ru

Corresponding Member of the RAS

Russian Federation, Saint-Petersburg

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