Thapsigargin, inhibitor of sarco-endoplasmic Ca2+-ATPase, effectively suppresses the expression of S100A4 in human breast cancer cell line
- Authors: Kotnova A.P.1, Lyanova B.M.1, Dukhanina E.A.1, Portseva T.N.1, Ilyin Y.V.1, Georgieva S.G.1, Stepchenko A.G.1, Pankratova E.V.1
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Affiliations:
- Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
- Issue: Vol 486, No 2 (2019)
- Pages: 255-257
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/13451
- DOI: https://doi.org/10.31857/S0869-56524862255-257
- ID: 13451
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Abstract
Thapsigargin, the SERCA ATPase inhibitor, effectively suppresses the expression of metastasis marker S100A4 in breast cancer cells MDA-MB231. It has been demonstrated that transcription of the S100A4 gene is controlled by Ca2+-signaling pathways. It has been shown that synthesis of S100A4 mRNA and protein in the MDA-MB231 cell line is effectively inhibited by thapsigargin at a concentration of 0.4-4 µM, while preserving cell survival. We assume that a change in gene transcription in response to the disruption of Ca2+ homeostasis plays a direct role in the remodeling of Ca2+-signaling pathways.
Keywords
About the authors
A. P. Kotnova
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Author for correspondence.
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
B. M. Lyanova
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
E. A. Dukhanina
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
T. N. Portseva
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
Yu. V. Ilyin
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Academician of the Russian Academy of Sciences
Russian Federation, 32, Vavilov street, Moscow, 119991S. G. Georgieva
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Corresponding Member of the Russian Academy of Sciences
Russian Federation, 32, Vavilov street, Moscow, 119991A. G. Stepchenko
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
E. V. Pankratova
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
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