Email this article Thapsigargin, inhibitor of sarco-endoplasmic Ca2+-ATPase, effectively suppresses the expression of S100A4 in human breast cancer cell line
- Authors: Kotnova A.P.1, Lyanova B.M.1, Dukhanina E.A.1, Portseva T.N.1, Ilyin Y.V.1, Georgieva S.G.1, Stepchenko A.G.1, Pankratova E.V.1
-
Affiliations:
- Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
- Issue: Vol 486, No 2 (2019)
- Pages: 255-257
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/13451
- DOI: https://doi.org/10.31857/S0869-56524862255-257
- ID: 13451
Cite item
Full Text
Abstract
Thapsigargin, the SERCA ATPase inhibitor, effectively suppresses the expression of metastasis marker S100A4 in breast cancer cells MDA-MB231. It has been demonstrated that transcription of the S100A4 gene is controlled by Ca2+-signaling pathways. It has been shown that synthesis of S100A4 mRNA and protein in the MDA-MB231 cell line is effectively inhibited by thapsigargin at a concentration of 0.4-4 µM, while preserving cell survival. We assume that a change in gene transcription in response to the disruption of Ca2+ homeostasis plays a direct role in the remodeling of Ca2+-signaling pathways.
Keywords
About the authors
A. P. Kotnova
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Author for correspondence.
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
B. M. Lyanova
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
E. A. Dukhanina
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
T. N. Portseva
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
Yu. V. Ilyin
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Academician of the Russian Academy of Sciences
Russian Federation, 32, Vavilov street, Moscow, 119991S. G. Georgieva
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Corresponding Member of the Russian Academy of Sciences
Russian Federation, 32, Vavilov street, Moscow, 119991A. G. Stepchenko
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
E. V. Pankratova
Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
Email: alina_kotnova@mail.ru
Russian Federation, 32, Vavilov street, Moscow, 119991
References
- Stewart R. L., Carpenter B. L., West D. S., Knifley T., Liu L., Wang C., Weiss H. L., Gal T. S., Durbin E. B., Arnold S. M., O’Connor K.L., Chen M. // Oncotarget. 2016. V. 7. № 23. P. 34630-34642.
- Helfman D. M., Kim E. J., Lukanidin E., Grigorian M. // Brit. J. Cancer. 2005. V. 92. № 11. P. 1955-1958.
- Garrett S. C., Varney K. M., Weber D. J., Bresnick A. R. // J. Biol. Chem. 2006. V. 281. № 2. P. 677-680.
- Schneider M., Hansen J. L., Sheikh S. P. // J. Mol. Med. 2008. V. 86. № 5. P. 507-522.
- Grigorian M., Ambartsumian N., Lukanidin E. // Curr. Mol. Med. 2008. V. 8. № 6. P. 492-496.
- Духанина Е. А., Лукьянова Т. И., Духанин А. С., Геор-гиева С. Г. // Бюл. эксперим. биологии и медицины. 2017. Т. 164. № 11. С. 614-616.
- Chemaly E. R., Troncone L., Lebeche D. // Cell Calcium. 2018. V. 69. P. 46-61.
- Духанина Е. А., Лукьянова Т. И., Романова Е. А., Духанин А. С., Сащенко Л. П. // Бюл. эксперим. биологии и медицины. 2008. Т. 145. № 1. С. 85-88.
- Seth M., Sumbilla C., Mullen S. P., Lewis D., Klein M. G., Hussain A., Soboloff J., Gill D. L., Inesi G. // Proc. Natl. Acad. Sci. USA. 2004. V. 101. № 47. P. 16683-16688.
- Lorenz S., Aust G., Krohn K. // Biochim. Biophys. Acta. 2013. V. 1833. № 12. P. 2703-2713.
- Malashkevich V. N., Dulyaninova N. G., Ramagopal U. A., Liriano M. A., Varney K. M., Knight D., Brenowitz M., Weber D. J., Almo S. C., Bresnick A. R. // Proc. Natl. Acad. Sci. USA. 2010. V. 107. № 19. P. 8605-8610.
- Andersen T. B., López C. Q., Manczak T., Martinez K., Simonsen H. T. // Molecules. 2015. V. 20. № 4. P. 6113-6127.
Supplementary files
