NSP protein encoded in negative NS RNA strand of influenza A virus induces cellular immune response in infected animals
- Authors: Zhirnov O.P.1,2, Isaeva E.I.1
-
Affiliations:
- Ivanovsky Institute of Virology, N.F.Gamaleya Federal Research Centre of Epidemiology and Microbiology
- Russian-German Academy of medical and social Sciences
- Issue: Vol 486, No 3 (2019)
- Pages: 389-394
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/13528
- DOI: https://doi.org/10.31857/S0869-56524863389-394
- ID: 13528
Cite item
Full Text
Abstract
Infection of mice with influenza A viruses led to the formation of clones of lymphocytes that specifically recognizes viral domains in the central zone of the NSP protein (amino acid positions 83-119). Computer analysis of the primary structure of the NSP protein showed the presence of T-cell epitopes in the central part of the NSP molecule. The findings indicate that the viral NSP gene is expressed in the infected animals and support the concept of the bipolar strategy (ambisense strategy) of the influenza A virus genome.
Keywords
About the authors
O. P. Zhirnov
Ivanovsky Institute of Virology, N.F.Gamaleya Federal Research Centre of Epidemiology and Microbiology; Russian-German Academy of medical and social Sciences
Author for correspondence.
Email: zhirnov@inbox.ru
Russian Federation, 16, Gamalei street, Moscow, 123098; 36-9, Novy Arbat street, Moscow, 121205
E. I. Isaeva
Ivanovsky Institute of Virology, N.F.Gamaleya Federal Research Centre of Epidemiology and Microbiology
Email: zhirnov@inbox.ru
Russian Federation, 16, Gamalei street, Moscow. 123098
References
- Dou D., Revol R., Östbye H., Wang H., Daniels R. Influenza A Virus Cell Entry, Replication, Virion Assembly and Movement // Front Immunol. 2018. V. 9. P. 1581. doi: 10.3389/fimmu.2018.01581
- Palese Baez M., Zazra J.J., Elliott R.M., Young J.F., Palese P. Nucleotide Sequence of the Influenza A/duck/Alberta/60/76 virus NS RNA: Conservation of the NS1/NS2 Overlapping Gene Structure in a Divergent Influenza Virus RNA Segment // Virology. 1981. V. 113. № 1. P. 397-402.
- Жирнов О.П., Поярков С.В., Воробьева И.В., Сафонова О.А., Малышев Н.А., Кленк Н.Д. Сегмент NS вирусов гриппа А содержит дополнительный ген NSP в позитивной ориентации // ДАН. 2007. Т. 414. № 4. С. 547-553. PubMed PMID: 17695319.
- Zhirnov O.P., Vorobjeva I.V., Saphonova O.A., Poyarkov S.V., Ovcharenko A.V., Anhlan D., Malyshev N.A. Structural and Evolutionary Characteristics of HA, NA, NS and M Genes of Clinical Influenza A/H3N2 Viruses Passaged in Human and Canine Cells // J. Clin. Virol. 2009. V. 45. № 4. P. 322-333. doi: 10.1016/j.jcv.2009.05.030
- Жирнов О.П., Акулич К.А., Липатова А.В., Усачев Е.В. Негативно-полярная вирионная РНК сегмента 8 (NS) вируса гриппа А способна транслировать in vitro новый вирусный белок // ДАН. 2017. Т. 473. № 4. С. 122-127. doi: 10.1134/S160767291 7020090
- Clifford M., Twigg J., Upton C. Evidence for a Novel Gene Associated with Human Influenza A viruses // Virol. J. 2009. V. 6. P. 198. DOI: 10.1186/ 1743-422X 6-198
- Gong Y.N., Chen G.W., Chen C.J., Kuo R.L., Shih S.R. Computational Analysis and Mapping of Novel Open Reading Frames in Influenza A Viruses // PLoS One. 2014. V. 9. № 12. e115016. doi: 10.1371/journal.pone.0115016
- Yang C.W., Chen M.F. Uncovering the Potential Pan Proteomes Encoded by Genomic Strand RNAs of Influenza A Viruses // PLoS One. 2016. V. 11. № 1. e0146936. doi: 10.1371/journal.pone. 0146936
- Sabath N., Morris J.S., Graur D. Is There a Twelfth Protein-Coding Gene in the Genome Of Influenza A? A Selection-Based Approach to the Detection of Overlapping Genes in Closely Related Sequences // J. Mol. Evol. 2011. V. 73. № 5/6. P. 305-315. doi: 10.1007/s00239-011-9477-9
- Jensen K.K., Andreatta M., Marcatili P., Buus S., Greenbaum J.A., Yan Z., Sette A., Peters B., Nielsen M. Improved Methods for predicting Peptide Binding Affinity to MHC Class II Molecules // Immunology. 2018. V. 154. № 3. P. 394-406. doi: 10.1111/imm.12889
- Bhasin M., Raghava G.P.S. A Hybrid Approach for Predicting Promiscuous MHC Class I restricted T Cell Epitopes // J. Biosci. 2006. V. 32. P. 31-42.
- Jameson B.A., Wolf H. The Antigenic Index: a Novel Algorithm for Predicting Antigenic Determinants // Comput. Appl. Biosci. 1988. V. 4. № 1. P. 181-186.
- Panthu B., Terrier O., Carron C., Traversier A., Corbin A., Balvay L., Lina B., Rosa-Calatrava M., Ohlmann T. The NS1 Protein from Influenza Virus Stimulates Translation Initiation by Enhancing Ribosome Recruitment to mRNAs // J. Mol. Biol. 2017. V. 429. № 21. P. 3334-3352. doi: 10.1016/j.jmb.2017.04.007
- Zhong W., Reche P.A., Lai C.C., Reinhold B., Reinherz E.L. Genome-Wide Characterization of a Viral Cytotoxic T Lymphocyte Epitope Repertoire // J. Biol. Chem. 2003. V. 278. № 46. P. 45 135-45 144.
- Nguyen M., Haenni A.L. Expression Strategies of Ambisense Viruses // Virus Res. 2003. V. 93. № 2. P. 141-150.