Email this article Encapsulation of neurotoxins - blockers of nicotinic acetylcholine receptors - in nanomaterials based on sulfated polysaccharides
- Authors: Nguyen T.D.1,2, Nguyen T.N.2,3, Nguyen K.C.1,2, Tran Q.N.1,2, Hoang A.N.1,2, Egorova N.S.4, Starkov V.G.4, Tsetlin V.I.4, Utkin Y.N.4
-
Affiliations:
- Institute of Applied Materials Science, Vietnam Academy of Science and Technology
- Graduate University of Science and Technology, Vietnam Academy of Science and Technology
- Tra Vinh University
- Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Issue: Vol 487, No 1 (2019)
- Pages: 102-106
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/15406
- DOI: https://doi.org/10.31857/S0869-56524871102-106
- ID: 15406
Cite item
Full Text
Abstract
The three-finger snake neurotoxins are selective antagonists of some nicotinic cholinergic receptor subtypes and are used to study these receptors. The peptide neurotoxin azemiopsin, recently isolated from the venom of Azemipos feae, is a selective muscle-type cholinergic receptor blocker. In order to reduce their toxicity and increase resistance under physiological conditions, we have encapsulated these toxins into nanomaterials. The study of nanomaterials after interaction with neurotoxins by the methods of transmission electron microscopy and dynamic light scattering revealed an increase in the size of nanoparticles, which indicates the inclusion of neurotoxins in nanomaterials.
Keywords
About the authors
Trung D. Nguyen
Institute of Applied Materials Science, Vietnam Academy of Science and Technology; Graduate University of Science and Technology, Vietnam Academy of Science and Technology
Email: yutkin@yandex.ru
Viet Nam, 01A, Thanh Loc 29 Street, Thanh Loc Ward, District 12, Ho Chi Minh City; 18, Hoang Quoc Viet, Cau Giay, Hanoi City
The N. Nguyen
Graduate University of Science and Technology, Vietnam Academy of Science and Technology; Tra Vinh University
Email: yutkin@yandex.ru
Viet Nam, 18, Hoang Quoc Viet, Cau Giay, Hanoi City; 126, Nguyen Thien Thanh St., Tra Vinh Province
Khoa C. Nguyen
Institute of Applied Materials Science, Vietnam Academy of Science and Technology; Graduate University of Science and Technology, Vietnam Academy of Science and Technology
Email: yutkin@yandex.ru
Viet Nam, 01A, Thanh Loc 29 Street, Thanh Loc Ward, District 12, Ho Chi Minh City; 18, Hoang Quoc Viet, Cau Giay, Hanoi City
Quyen N. Tran
Institute of Applied Materials Science, Vietnam Academy of Science and Technology; Graduate University of Science and Technology, Vietnam Academy of Science and Technology
Email: yutkin@yandex.ru
Viet Nam, 01A, Thanh Loc 29 Street, Thanh Loc Ward, District 12, Ho Chi Minh City; 18, Hoang Quoc Viet, Cau Giay, Hanoi City
Anh N. Hoang
Institute of Applied Materials Science, Vietnam Academy of Science and Technology; Graduate University of Science and Technology, Vietnam Academy of Science and Technology
Email: yutkin@yandex.ru
Viet Nam, 01A, Thanh Loc 29 Street, Thanh Loc Ward, District 12, Ho Chi Minh City; 18, Hoang Quoc Viet, Cau Giay, Hanoi City
N. S. Egorova
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: yutkin@yandex.ru
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997
V. G. Starkov
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: yutkin@yandex.ru
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997
V. I. Tsetlin
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: yutkin@yandex.ru
Corresponding Member of the Russian Academy of Sciences
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997Yu. N. Utkin
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Author for correspondence.
Email: yutkin@yandex.ru
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997
References
- Dutertre S., Nicke A., Tsetlin V. I. Nicotinic Acetylcholine Receptor Inhibitors Derived from Snake and Snail Venoms // Neuropharmacology. 2017. V. 127. P. 196-223. doi: 10.1016/j.neuropharm.2017.06.011.
- Utkin Y. N., Weise C., Kasheverov I. E., Andreeva T. V., Kryukova E. V., Zhmak M. N., Starkov V. G., Hoang N. A., Bertrand D., Ramerstorfer J., Sieghart W., Thompson A. J., Lummis S. C., Tsetlin V. I. Azemiopsin from Azemiops Feae Viper Venom, a Novel Polypeptide Ligand of Nicotinic Acetylcholine Receptor // J. Biol. Chem. 2012. V. 287. № 32. P. 27 079-27 086. doi: 10.1074/jbc.M112.363051.
- Shelukhina I. V., Zhmak M. N., Lobanov A. V., Ivanov I. A., Garifulina A. I., Kravchenko I. N., Rasskazova E. A., Salmova M. A., Tukhovskaya E. A., Rykov V. A., Slashcheva G. A., Egorova N. S., Muzyka I. S., Tsetlin V. I., Utkin Y. N. Azemiopsin, a Selective Peptide Antagonist of Muscle Nicotinic Acetylcholine Receptor: Preclinical Evaluation as a Local Muscle Relaxant // Toxins (Basel). 2018. V. 10. № 1. pii: E34. doi: 10.3390/toxins10010034.
- McClements D. J. Encapsulation, Protection, and Delivery of Bioactive Proteins and Peptides Using Nanoparticle and Microparticle Systems: A Review // Adv. Colloid Interface Sci. 2018. V. 253. P. 1-22. doi: 10.1016/j.cis.2018.02.002.
- Sharma G., Sharma A. R., Nam J. S., Doss G. P., Lee S. S., Chakraborty C. Nanoparticle Based Insulin Delivery System: The Next Generation Efficient Therapy for Type 1 Diabetes // J. Nanobiotechnology. 2015. V. 13. P. 74. doi: 10.1186/s12951-015-0136-y.
- Jeong I., Kim B. S., Lee H., Lee K. M., Shim I., Kang S. K., Yin C. S., Hahm D. H. Prolonged Analgesic Effect of PLGA Encapsulated Bee Venom on Formalin-Induced Pain in Rats // Int. J. Pharm. 2009. V. 380. № 1/2. P. 62-66. doi: 10.1016/j.ijpharm.2009.06.034.
- Ngoc The Nguyen, Thi Hiep Nguyen, Minh Thanh Vu, Van Thu Le, Xuan Anh Nguyen, Tram Chau Nguyen, Thi Bich Tram Nguyen. Novel Amphiphilic Heparin-Pluronic P123 Copolymers Exhibiting a Great Potential for Cisplatin Delivery // J. Mater. Sci. 2018. V. 53. P. 12 692. doi: 10.1007/s10853-018-2569-9.
- Cuu Khoa Nguyen, Ngoc Quyen Tran, Thi Phuong Nguyen, Dai Hai Nguyen. Biocompatible Nanomaterials Based on Dendrimers, Hydrogels and Hydrogel Nanocomposites for Use in Biomedicine // Adv. Nat. Sci.: Nanosci. Nanotechnol. 2017. V. 8. 015001. 10 p. doi: 10.1088/2043-6254/8/1/015001.
- Shao N., Su Y., Hu J., Zhang J., Zhang H., Cheng Y. Comparison of Generation 3 Polyamidoamine Dendrimer and Generation 4 Polypropylenimine Dendrimer on Drug Loading, Complex Structure, Release Behavior, and Cytotoxicity // Int. J. Nanomedicine. 2011. V. 6. P. 3361-3372. doi: 10.2147/IJN.S27028.
- Yang Q., Zhao C., Zhao J., Ye Y. Photoresponsive Nanocapsulation of Cobra Neurotoxin and Enhancement of Its Central Analgesic Effects under Red Light // Int. J. Nanomedicine. 2017. V. 12. P. 3463-3470. doi: 10.2147/IJN.S132510.
Supplementary files
