Cardiotoxins from cobra Naja oxiana change the force of contraction and the character of rhythmoinotropic phenomena in the rat myocardium

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Abstract

The study of the influence of cobra Naja oxiana cardiotoxins on the contractility of the rat papillary muscles and its rhythm-inotropic characteristics has that the presence of toxins induces a slight contractility decrease in the stimulation frequency range up to 0,1 Hz. In the stimulation frequency range from 0,1 to 0,5 Hz a positive inotropic effect is found. However, the positive inotropic effect is replaced by a negative one with further increase in the frequency up to 3 Hz. In the presence of cardiotoxins, the positive force-frequency relationship in the region of 1-3 Hz, characteristic of healthy rat myocardium, disappears and relationship becomes completely negative. L‑type calcium channel blocker nifedipine does not affect the changes induced by toxins, while a high concentration (10 mM) of calcium prevents the effects of cardiotoxins on the muscle. The results obtained show that the impairment of the force-frequency relationship occurs long before the development of irreversible damage in the myocardium and may be the first sign of the pathological action of cardiotoxins.

About the authors

A. S. Averin

Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center of Biological Research”, Pushchino Branch of the Russian Academy of Sciences

Author for correspondence.
Email: averin.icb@gmail.ru
Russian Federation, 3, Institutskaya street, Pushchino, Moscow Region, 142290

M. E. Astashev

Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center of Biological Research”, Pushchino Branch of the Russian Academy of Sciences

Email: averin.icb@gmail.ru
Russian Federation, 3, Institutskaya street, Pushchino, Moscow Region, 142290

T. V. Andreeva

Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: averin.icb@gmail.ru
Russian Federation, 16/10, Miklukho-Maklaya street, Moscow, 117997

V. I. Tsetlin

Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: averin.icb@gmail.ru

Corresponding Member of the Russian Academy of Sciences

Russian Federation, 16/10, Miklukho-Maklaya street, Moscow, 117997

Yu. N. Utkin

Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: yutkin@yandex.ru
Russian Federation, 16/10, Miklukho-Maklaya street, Moscow, 117997

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