Cardiotoxins from cobra Naja oxiana change the force of contraction and the character of rhythmoinotropic phenomena in the rat myocardium
- Authors: Averin A.S.1, Astashev M.E.1, Andreeva T.V.2, Tsetlin V.I.2, Utkin Y.N.2
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Affiliations:
- Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center of Biological Research”, Pushchino Branch of the Russian Academy of Sciences
- Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Issue: Vol 487, No 5 (2019)
- Pages: 578-583
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/15912
- DOI: https://doi.org/10.31857/S0869-56524875578-583
- ID: 15912
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Full Text
Abstract
The study of the influence of cobra Naja oxiana cardiotoxins on the contractility of the rat papillary muscles and its rhythm-inotropic characteristics has that the presence of toxins induces a slight contractility decrease in the stimulation frequency range up to 0,1 Hz. In the stimulation frequency range from 0,1 to 0,5 Hz a positive inotropic effect is found. However, the positive inotropic effect is replaced by a negative one with further increase in the frequency up to 3 Hz. In the presence of cardiotoxins, the positive force-frequency relationship in the region of 1-3 Hz, characteristic of healthy rat myocardium, disappears and relationship becomes completely negative. L‑type calcium channel blocker nifedipine does not affect the changes induced by toxins, while a high concentration (10 mM) of calcium prevents the effects of cardiotoxins on the muscle. The results obtained show that the impairment of the force-frequency relationship occurs long before the development of irreversible damage in the myocardium and may be the first sign of the pathological action of cardiotoxins.
About the authors
A. S. Averin
Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center of Biological Research”, Pushchino Branch of the Russian Academy of Sciences
Author for correspondence.
Email: averin.icb@gmail.ru
Russian Federation, 3, Institutskaya street, Pushchino, Moscow Region, 142290
M. E. Astashev
Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center of Biological Research”, Pushchino Branch of the Russian Academy of Sciences
Email: averin.icb@gmail.ru
Russian Federation, 3, Institutskaya street, Pushchino, Moscow Region, 142290
T. V. Andreeva
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: averin.icb@gmail.ru
Russian Federation, 16/10, Miklukho-Maklaya street, Moscow, 117997
V. I. Tsetlin
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: averin.icb@gmail.ru
Corresponding Member of the Russian Academy of Sciences
Russian Federation, 16/10, Miklukho-Maklaya street, Moscow, 117997Yu. N. Utkin
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: yutkin@yandex.ru
Russian Federation, 16/10, Miklukho-Maklaya street, Moscow, 117997
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