Email this article Protein-partners of selenoprotein SELM and the role of selenium compounds in regulation of its expression in human cancer cells
- Authors: Varlamova E.G.1, Goltyaev M.V.1, Fesenko E.E.1
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Affiliations:
- Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center of Biological Research”, Pushchino Branch of the Russian Academy of Sciences
- Issue: Vol 488, No 2 (2019)
- Pages: 212-216
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/16098
- DOI: https://doi.org/10.31857/S0869-56524882212-216
- ID: 16098
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Abstract
The search of potential partners of human SELM in lysates of two cancer cell lines HT-1080 (fibrosarcoma) and MCF-7 (breast adenocarcinoma) was carried out. Two cytoplasmic actin isoforms: cytoplasmic actin 1 (cytoskeleton b-actin) and cytoplasmic actin 2 (cytoskeletal g-actin) was identified as partners. In addition, the influence of two widely used antitumor selenium compounds (sodium selenite and methylseleninic acid) on the expression SELM in cancer cells was studied. According to the results obtained by real-time PCR and Western blotting, we was concluded that 1 µM and 10 µM sodium selenite was not affected on the expression SELM in fibrosarcoma cells, whereas in breast adenocarcinoma cells 1 µM sodium selenite slightly increased of expression and 10 µM resulted in a significant decrease (about 2 times). Methylseleninic acid in both cancer cell lines increased the expression of SELM gene, the most pronounced effect was observed when fibrosarcoma cells were treated with 10 µM MSC (increased expression of the hSelm gene by almost 4 times).
About the authors
E. G. Varlamova
Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center of Biological Research”, Pushchino Branch of the Russian Academy of Sciences
Author for correspondence.
Email: 1928lv@mail.ru
Russian Federation, 3, Institutskaya street, Pushchino, Moscow Region, 142290
M. V. Goltyaev
Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center of Biological Research”, Pushchino Branch of the Russian Academy of Sciences
Email: 1928lv@mail.ru
Russian Federation, 3, Institutskaya street, Pushchino, Moscow Region, 142290
E. E. Fesenko
Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center of Biological Research”, Pushchino Branch of the Russian Academy of Sciences
Email: 1928lv@mail.ru
Corresponding Member of the Russian Academy of Sciences
Russian Federation, 3, Institutskaya street, Pushchino, Moscow Region, 142290References
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