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An approach to better estimation of the intial velocity of enzyme reactions
- Authors: Malygina N.M.1,2, Petrova T.A.1, Lianguzov A.Y.1, Ivanov A.M.2
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Affiliations:
- Saint-Petersburg State University
- Military medical academy of S.M. Kirov
- Issue: Vol 488, No 2 (2019)
- Pages: 225-228
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/16114
- DOI: https://doi.org/10.31857/S0869-56524882225-228
- ID: 16114
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Abstract
A new algorithm for computation of initial velocity of enzyme reaction at the time zero is proposed. This algorithm makes it possible to reduce systematic error of measurements to the minimum, to estimate reaction velocity in testing samples regardless of the enzyme activity levels and to minimize assay time. The study is illustrated by an example of salivary alpha-amylase and standard reagent kit. The algorithm should not be applied if conjugated enzyme systems are used because there is a long initial lag-phase in the kinetic curve.
About the authors
N. M. Malygina
Saint-Petersburg State University; Military medical academy of S.M. Kirov
Email: andrey.lyanguzov@spbu.ru
Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034; 6G, Akademika Lebedeva street, Saint-Petersburg, 194044
T. A. Petrova
Saint-Petersburg State University
Email: andrey.lyanguzov@spbu.ru
Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034
A. Y. Lianguzov
Saint-Petersburg State University
Author for correspondence.
Email: andrey.lyanguzov@spbu.ru
Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034
A. M. Ivanov
Military medical academy of S.M. Kirov
Email: andrey.lyanguzov@spbu.ru
Corresponding Member of the Russian Academy of Sciences
Russian Federation, 6G, Akademika Lebedeva street, Saint-Petersburg, 194044References
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- Cornish-Bowden A. Fundamentals of Enzyme Kinetics. L.: Portland Press, 1995. 343 p.
- R Project: https://www.r-project.org/
- Lyangusov A.Yu., Petrova T.A., Stefanov V.E. // Dokl. Biochem. and Biophys. 2009. V. 424. № 1. P. 49-52.
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