An approach to better estimation of the intial velocity of enzyme reactions

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Abstract

A new algorithm for computation of initial velocity of enzyme reaction at the time zero is proposed. This algorithm makes it possible to reduce systematic error of measurements to the minimum, to estimate reaction velocity in testing samples regardless of the enzyme activity levels and to minimize assay time. The study is illustrated by an example of salivary alpha-amylase and standard reagent kit. The algorithm should not be applied if conjugated enzyme systems are used because there is a long initial lag-phase in the kinetic curve.

About the authors

N. M. Malygina

Saint-Petersburg State University; Military medical academy of S.M. Kirov

Email: andrey.lyanguzov@spbu.ru
Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034; 6G, Akademika Lebedeva street, Saint-Petersburg, 194044

T. A. Petrova

Saint-Petersburg State University

Email: andrey.lyanguzov@spbu.ru
Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034

A. Y. Lianguzov

Saint-Petersburg State University

Author for correspondence.
Email: andrey.lyanguzov@spbu.ru
Russian Federation, 7/9, Universitetskaya embankment, Saint-Petersburg, 199034

A. M. Ivanov

Military medical academy of S.M. Kirov

Email: andrey.lyanguzov@spbu.ru

Corresponding Member of the Russian Academy of Sciences

Russian Federation, 6G, Akademika Lebedeva street, Saint-Petersburg, 194044

References

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  2. Foo Y. A., Bais R. // Clin. Chim. Acta. 1998. V. 272. P. 137-147.
  3. Cornish-Bowden A. Fundamentals of Enzyme Kinetics. L.: Portland Press, 1995. 343 p.
  4. R Project: https://www.r-project.org/
  5. Lyangusov A.Yu., Petrova T.A., Stefanov V.E. // Dokl. Biochem. and Biophys. 2009. V. 424. № 1. P. 49-52.

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