Effects of gamma-radiation on DNA damage in onion (Allium cepa L.) seedlings

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Abstract


The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0,1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0,1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose gamma-radiation on Allium cepa seedlings.


About the authors

A. Ya. Bolsunovsky

Institute of Biophysics of the Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”

Author for correspondence.
Email: radecol@ibp.ru

Russian Federation, 50, Akademgorodok, Krasnoyarsk, 660036

D. V. Dementyev

Institute of Biophysics of the Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”

Email: radecol@ibp.ru

Russian Federation, 50, Akademgorodok, Krasnoyarsk, 660036

T. S. Frolova

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science; Novosibirsk State University

Email: radecol@ibp.ru

Russian Federation, 10, Lavrentjev avenue, Novosibirsk, 630090; 1, Pirogova street, Novosibirsk, 630090

E. A. Trofimova

Institute of Biophysics of the Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”

Email: radecol@ibp.ru

Russian Federation, 50, Akademgorodok, Krasnoyarsk, 660036

E. M. Iniatkina

Institute of Biophysics of the Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”

Email: radecol@ibp.ru

Russian Federation, 50, Akademgorodok, Krasnoyarsk, 660036

S. A. Vasilyev

Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences

Email: radecol@ibp.ru

Russian Federation, 10, Ushaiki River Embankment, Tomsk, 634050

O. I. Sinitsyna

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Science; Novosibirsk State University

Email: radecol@ibp.ru

Russian Federation, 10, Lavrentjev avenue, Novosibirsk, 630090; 1, Pirogova street, Novosibirsk, 630090

References

  1. Болсуновский А.Я., Суковатый А.Г. // Радиационная биология. Радиоэкология. 2004. Т. 44. № 3. С. 393-398.
  2. Bolsunovsky A. // Chem. Ecol. 2010. V. 26. № 6. P. 401-409.
  3. Bolsunovsky A., Melgunov M., Chuguevskii A., Lind O.C., Salbu B. // Sci. Repts. 2017. № 7. Article number: 11132. Р. 1-10.
  4. Bolsunovsky A., Frolova T., Dementyev D., Sinitsyna O. // Ecotoxic and Environ. Safety. 2016. V. 134. Pt 1. P. 233-238.
  5. Болсуновский А.Я., Дементьев Д.В., Трофимова Е.А., Зотина Т.А. // ДАН. 2017. Т. 475. № 1. С. 113-119.
  6. Болсуновский А.Я., Дементьев Д.В., Трофимова Е.А., Иняткина Е.М., Кладко Ю.В., Петриченков М.В. // ДАН. 2018. Т. 481. № 1. С. 99-103.
  7. Olive P.L. // Int. J. Radiation Biology. 1999. V. 75. № 4. P. 395-405.
  8. Collins A.R. // Molecular Biotechn. 2004. V. 26. P. 249-261.
  9. Gichner T., Patková Z., Száková J., Demnerová K. // Mutation Research. 2004. V. 559. P. 49-57.
  10. Dona M., Ventura L., Macovei A., Confalonieri M., Savio M., Giovannini A., Carbonera D., Balestrazzi A. // J. Plant Physiology. 2013. V. 170. P. 780-787.
  11. Koppen G., Cerda H. // LWT-Food Science and Technology. 1997. V. 30. P. 452-457.
  12. Tae Ho Ryua, Jin Kyu Kima, Jeong-Il Kimb, Jin-Hong Kima // J. Environ. Radioactivity. 2018. V. 181. P. 94-101.
  13. Saghirzadeh M., Gharaati M.R., Mohammadi Sh., Ghiassi-Nejad M. // J. Environ. Radioactivity. 2008. V. 99. P. 1698-1702.
  14. Torudd J., Protopopova M., Sarimov R., Nygren J., Eriksson S., Markova E., Chovanec M., Selivanova G., Belyaev I.Y. // Int. J. Radiat. Biol. 2005. V. 81. P. 125-138.

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