Recombinant analogue of the human protein SLURP-1 inhibits the growth of multicellular carcinoma cell spheroids
- Authors: Bychkov M.L.1, Shulepko M.A.1, Shlepova O.V.1,2, Lyukmanova E.N.1,2, Kirpichnikov M.P.1,3
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Affiliations:
- Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Moscow Institute of Physics and Technology
- Lomonosov Moscow State University
- Issue: Vol 489, No 5 (2019)
- Pages: 517-520
- Section: Biochemistry, biophysics, molecular biology
- URL: https://journals.eco-vector.com/0869-5652/article/view/18850
- DOI: https://doi.org/10.31857/S0869-56524895517-520
- ID: 18850
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Abstract
The present study showed that the recombinant analogue of SLURP‑1 effectively inhibits the growth of a 3D model of tumors - multicellular spheroids from human epidermoid carcinoma A431 cells and human lung adenocarcinoma A549 cells. The combined application of SLURP‑1 with gefitinib (inhibitor of epidermal growth factor receptor (EGFR)) leads to the synergistic antiproliferative effect on spheroids from A431 cells. The results obtained suggest the possibility for design of first-in-class anticancer drugs based on recombinant SLURP‑1.
About the authors
M. L. Bychkov
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: mikhailshulepko@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997
M. A. Shulepko
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Author for correspondence.
Email: mikhailshulepko@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997
O. V. Shlepova
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Moscow Institute of Physics and Technology
Email: mikhailshulepko@gmail.com
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997; 9, Institutskij, Dolgoprudny, Moscow region, 141701
E. N. Lyukmanova
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Moscow Institute of Physics and Technology
Email: ekaterina-lyukmanova@yandex.ru
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997; 9, Institutskij, Dolgoprudny, Moscow region, 141701
M. P. Kirpichnikov
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Lomonosov Moscow State University
Email: mikhailshulepko@gmail.com
Academician of the Russian Academy of Sciences
Russian Federation, 16/10, Miklukho-Maklaya Street, Moscow, 117997; 1, Leninskie gory, Moscow, 119991References
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