The generation of the human mab RabD4 specific to the rabies virus glycoprotein and characterization thereof

Е. N. Ilina; Ильина Е. Н.; E. V. Solopova; Солопова Е. В.; Т. К. Aliev; Алиев Т. К.; М. V. Larina; Ларина М. В.; D. S. Balabashin; Балабашин Д. С.; N. Е. Varlamov; Варламов Н. Е.; D. А. Dolgikh; Долгих Д. А.; P. G. Sveshnikov; Свешников П. Г.; М. P. Kirpichnikov; Кирпичников М. П.

doi:10.31857/S0869-56524853370-373

The generation of the human mab RabD4 specific to the rabies virus glycoprotein and characterization thereof

Authors: Ilina Е.N.¹, Solopova E.V.², Aliev Т.К.¹, Larina М.V.³, Balabashin D.S.³, Varlamov N.Е.², Dolgikh D.А.¹^,3, Sveshnikov P.G.², Kirpichnikov М.P.¹^,3
Affiliations:
1. Lomonosov Moscow State University
2. All-Russian Scientific Center for Molecular Diagnostics and Treatment
3. Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science
Issue: Vol 485, No 3 (2019)
Pages: 370-373
Section: Biochemistry, biophysics, molecular biology
URL: https://journals.eco-vector.com/0869-5652/article/view/12889
DOI: https://doi.org/10.31857/S0869-56524853370-373
ID: 12889

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Abstract

We generated a novel human neutralizing human mAb RabD4 against rabies virus glycoprotein using in vitro stimulation human peripheral B cells produced from immunized donor. It was revealed that the human mAb RabD4 demonstrated high antigen-binding activity and virus-neutralizing activity in the FAVN test with the CVS-11 rabies virus.

Keywords

rabies virus, monoclonal antibodies, glycoprotein, FAVN, post-exposure prophylaxis

About the authors

Е. N. Ilina

Lomonosov Moscow State University

Author for correspondence.
Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow

E. V. Solopova

All-Russian Scientific Center for Molecular Diagnostics and Treatment

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow

Т. К. Aliev

Lomonosov Moscow State University

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow

М. V. Larina

Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow

D. S. Balabashin

Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow

N. Е. Varlamov

All-Russian Scientific Center for Molecular Diagnostics and Treatment

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow

D. А. Dolgikh

Lomonosov Moscow State University;Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow;Moscow

P. G. Sveshnikov

All-Russian Scientific Center for Molecular Diagnostics and Treatment

Email: Ekaterina.iljina.7@yandex.ru
Russian Federation, Moscow

М. P. Kirpichnikov

Lomonosov Moscow State University;Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science

Email: Ekaterina.iljina.7@yandex.ru

academician of the RAS

Russian Federation, Moscow;Moscow

References

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Ilina E. N., Solopova O. N., Balabashin D. S., Larina M. V., Aliev T. K., Grebennikova T. V., Losich M. A., Zaykova O. N., Sveshnikov P. G., Dolgikh D. A., Kirpi- chnikov M. P. // Russ. J. Bioorg. Chem. 2019. V. 45. P. 1-11.
de Kruif J., Bakker A. B., Marissen W. E., Kramer R. A., Throsby M., Rupprecht C. E., Goudsmit J. // Annu. Rev. Med. 2007. V. 58. P. 359-368.
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