Investigation of release kinetics of doxorubicin from drug carrier based on Fe(0) microparticles under influence of ultrasound irradiation and different values of pH


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Abstract

Aim. To obtain new drug carrier for doxorubicin based on modified Fe(0) microparticles and evaluate its release kinetics under influence of different values of pH and ultrasound irradiation. Material and methods. Size and zeta-potential of microparticles were determined on Zetasizer Nano ZS. Surface modification (covalent binding of residues of benzoic acid) was confirmed by FTIR spectroscopy. Encapsulation efficiency (EE) and loading capacity (LC) of doxorubicin (DOX) was determined by UV spectroscopy (480 nm). Release studies were carried out in Stuart SI 500 incubator at a constant temperature (37 °C), stirring rate (100 rpm) and different pH values (3.3; 5.5; 7.4). For investigation of influence of ultrasound (US) irradiation on the release kinetics ultrasound field with frequency and power 75 kHz and 2 W/cm2 respectively was used. Ultrasonic bath Elmasonic S10H was used as a source of ultrasound irradiation. Results and discussions. Size and zeta-potential of Fe-CS-DOX conjugate were 4.43 and -9.07 respectively. Loading capacity of doxorubicin was 0.54 mg/mg. Percentage of released drug with and without US irradiation were 96 and 18% respectively (in 12 hours after starting of the experiment). Conclusion. In this study, the release of doxorubicin from drug carrier, based on Fe (0) microparticles at different pH values was investigated and the influence of ultrasound irradiation on the release kinetics was confirmed. In 12 hours after starting of the release, the amount of released drug was increased more than 4 times. So, the obtained conjugate Fe-CS-DOX leaves great promise for its further use as a drug carrier.

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About the authors

S. S Vlasov

Tomsk Polytechnic University; Siberian State Medical University

Email: unreal800@gmail.com
Post-graduate Student, Research School of Chemistry and Applied Biomedical Sciences; Production Engineer, Technology Implementation Centre, Central Scientific Research Laboratory

A. Di Martino

Tomsk Polytechnic University; Tomas Bata University

Ph.D., Research Scientist, Research School of Chemistry and Applied Biomedical Sciences; Junior Research Scientist, Centre of Polymer Systems

M. S Yusubov

Tomsk Polytechnic University; Siberian State Medical University

Dr.Sc. (Chem.), Professor, Headmaster of Research School of Chemistry and Applied Biomedical Sciences; Chief Research Scientist, Central Scientific Research Laboratory

A. M Guryev

Siberian State Medical University

Dr.Sc. (Pharm.), Head of Technology Implementation Centre, Central Scientific Research Laboratory

S. V Krivoschekov

Siberian State Medical University

Junior Research Scientist, Central Scientific Research Laboratory

E. V Sviridova

Tomsk Polytechnic University

Post-graduate Student, Research School of Chemistry and Applied Biomedical Sciences

P. S Postnikov

Tomsk Polytechnic University M.V. Belousov

Ph.D. (Chem.), Associate Professor, Research School of Chemistry and Applied Biomedical Sciences

M. V Belousov

Siberian State Medical University

Dr. Sc. (Pharm), Head of Department of Pharmaceutical Analysis

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