Redox regulation of apoptosis and proliferation in psoriasis patients using omega-3-PUFA-containing drugs by thiol-disulfide exchange

Introduction. The relevance of the topic is due to the wide prevalence of psoriasis, which is characterized as increase in the intensity of keratinocyte proliferation. One of the factors contributing to the initiation of proliferation processes is the overreaction of the antioxidant system to inflammation, accompanied by a decrease in the intensity of free radical oxidation.

Material and Methods. The study included patients with an established diagnosis of psoriasis vulgaris, divided into three groups of 15 people, who along with standard therapy received the biologically active supplement "Omega-3 35%". The control group consisted of 15 healthy people. The antioxidant status was assessed by the state of the enzymatic and non-enzymatic link: the activity of catalase and superoxide dismutase of blood hemolysate and their ratio, the concentration of total HS-groups in plasma; the content of end products of lipid peroxidation – by the concentration of malondialdehyde in plasma. The state of apoptosis was determined by flow cytometry, the intensity of proliferation – by clinical manifestations and the PASI index.

Purpose of the work – to study the effect of omega-3 polyunsaturated fatty acids in different doses.

Results. It has been shown that in patients with psoriasis, when added to the standard therapy "Omega-3 35%" at a dose of 3450 mg/day, divided into three doses of 1150 mg at three times a day, an antioxidant effect is manifested, instead of the expected pro-oxidant clinical improvement is insignificant (the decrease in the PASI index is less than 25%), and at a dose of 2640 mg/day once a day for a month, a moderate pro-oxidant effect is observed, which is necessary to reduce excess proliferation, and increase in the intensity of apoptosis, these changes are accompanied by clinical improvement and a decrease in the PASI index by 37% compared to the baseline.

Conclusions. It has been shown that in patients with psoriasis, a shift in redox equilibrium towards oxidized equivalents is necessary to obtain positive clinical dynamics. It is assumed that redox regulation with the addition of omega-3 PUFAs occurs due to a short-term increase in oxidative processes and is realized through thiold-disulfide exchange.