Elemental profiling of Trifolia leaves (Menyanthes trifoliata L.) by ICP-MS method
- Authors: Babeshina L.G.1, Pinigina A.Y.1,2, Tyurina E.V.2, Sotskova L.А.2, Paskar N.G.3
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Affiliations:
- Moscow University "Synergy"
- Moscow Medical University "Reaviz"
- I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation
- Issue: Vol 28, No 6 (2025)
- Pages: 109-120
- Section: Pharmaceutical chemistry
- URL: https://journals.eco-vector.com/1560-9596/article/view/686233
- DOI: https://doi.org/10.29296/25877313-2025-06-14
- ID: 686233
Cite item
Abstract
Introduction. The need for systematic studies of the elemental composition of Menyanthes trifoliata is due to the need to ensure the quality and safety of pharmacopoeial raw materials in accordance with modern requirements.
Aim. To carry out a analysis of the elemental composition of the leaves of Menyanthes trifoliata L., growing in the conditions of the swamp ecosystem of the Moscow region, using the ICP-MS method to assess compliance with the requirements of the GF RF, to identify the features of the accumulation of elements in specific environmental conditions.
Matherial and Methods. M. trifoliata leaf samples were collected in July 2022 from bog ecosystem in Moscow region (55°86′N, 37°16′E). Sample preparation was performed according to OFS.1.1.0005.15. GF RF XV. Quantitative determination of 62 elements was performed by ICP-MS using ELAN DRC-e instrument. Statistical analysis included Pearson correlation analysis and cluster analysis.
Results. Dominant concentrations of macroelements K > Ca > P > Mg > Na were established. Among essential microelements, Mn (215 μg/g) > Fe (111 μg/g) > Zn (53.2 μg/g) > Cu (9.77 μg/g) prevailed. The content of As (0.12 μg/g), Hg (0.015 μg/g) and Pb (0.16 μg/g) is below the maximum permissible concentrations of the Russian Pharmacopoeia by 4.2, 6.7 and 37.5 times, respectively. Cd concentration (2.87 μg/g) exceeds Russian Pharmacopoeia standards (1.0 μg/g) by 2.87 times. Correlation analysis revealed significant relationships between Ca and Sr (r=0.81; p<0.01), Mg and Sr (r=0,55; p<0,05), negative correlation between K and Al (r=–0.54; p<0.05).
Conclusion. For the first time, 62-element profiling of M. trifoliata in bog conditions was performed. Cd content exceedance excludes the use of the studied sample for drug production. The identified correlations between elements reflect bioaccumulation features in specific ecological conditions of bog ecosystems.
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About the authors
L. G. Babeshina
Moscow University "Synergy"
Author for correspondence.
Email: lbabeshina@yandex.ru
ORCID iD: 0000-0002-2028-0316
SPIN-code: 6738-4841
Dr.Sc. (Biol.), Associate Professor, Professor of the Department of General Biology and Pharmacy
Russian Federation, 80 b/3 Leningradsky Prospekt, Moscow, 125315A. Y. Pinigina
Moscow University "Synergy"; Moscow Medical University "Reaviz"
Email: Annapin83@mail.ru
ORCID iD: 0000-0003-1126-934X
SPIN-code: 8797-9268
Senior Lecturer at the Department of Nursing Technologies; Senior Lecturer at the Department of Internal Medicine at the Moscow Medical University
Russian Federation, 80 b/3 Leningradsky Prospekt, Moscow, 125315; 27/2, Trade Union street, Moscow, 117418E. V. Tyurina
Moscow Medical University "Reaviz"
Email: djun_25@mail.ru
ORCID iD: 0009-0001-7592-9952
Student of the Faculty of Medicine
Russian Federation, 27/2, Trade Union street, Moscow, 117418L. А. Sotskova
Moscow Medical University "Reaviz"
Email: Lyubavalev@mail.ru
ORCID iD: 0009-0006-1979-0665
Student of the Faculty of Medicine
Russian Federation, 27/2, Trade Union street, Moscow, 117418N. G. Paskar
I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation
Email: nnikpaskar@yandex.ru
ORCID iD: 0009-0002-6656-6667
SPIN-code: 8269-0440
Student of the A.P. Nelyubin Institute of Pharmacy
Russian Federation, 8/2, Trubetskaya str., Moscow, 119991References
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