Antioxidant and energy protective effects of hepatoprotective phytopolyextract in acute toxic hepatitis

Relevance. The widespread of conditions associated with the liver damage sets the demand for the development of new hepatoprotective agents. Their pharmacotherapeutic activity is mediated through the enhancement of hepatocytes' resistance to pathological processes, fastened regeneration, and the inhibition of pathogenetic syndromes of liver damage. Aim. The aim of our study is to determine the mechanisms of hepatoprotective and antioxidant ability along with the ability to correct the bioenergetic deficit in hepatocytes of the complex plant supplement comprised of Hypecoum erectum L.; Hedysarum alpinum L.; Glycyrrhiza Uralensis Fisch.; Calendula officinalis L.; Scutellaria baicalensis Georgi. in a series of experiments with Wistar line white rats as subjects. Material and methods. To assess peroxidation intensity detection of malondialdehyde in homogenized rat liver samples. Concentrations of glutathione (GSH) in blood and catalase in liver in conjunction with glutathione peroxidase activity in liver were used to evaluate the activity of the antioxidant defense system while ATP, lactate and pyruvate served as indicators of energy metabolism. Results. The complex plant supplement used in dose of 100 mg/kg demonstrated the most pronounced positive effect on antioxidant system and significantly lowered lipid peroxidation intensity while in the groups of 200 mg/kg and 300 mg/kg the effects were not as drastic and were proportionate to that of the comparison drug Karsil. Conclusions. Thus, determining the effects of the complex dry phytoextract with hepatoprotective properties in the rat model of experimental hepatitis the correction of oxidative stress, the normalization of the balance of redox reactions in the body, and the energy-protective effect in toxic liver damage were established.

complex herbal supplement, hepatoprotective effect, experimental hepatitis, antioxidant activity, energy metabolism