Relationship of the levels of tryptofan metabolites with adipokines and myokines in patients with various obesity phenotypes

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Relevance. In the last 10 years, there has been an active study of the system of conjugation of the metabolism of a macroorganism and its microbiome. The microbiotic conversion of tryptophan into biologically active signaling molecules is a potential regulatory mechanism by which the intestinal microbiota can change the metabolism of both intestinal cells and the entire macroorganism. However, the content of tryptophan metabolism metabolites in patients with metabolically healthy (MHO) and metabolically unhealthy obesity (MUHO), as well as the relationship of these metabolites with adipokines and myokines, has not yet been studied.

Purpose of the study. To study the content of metabolites of tryptophan metabolism in the blood serum of obese patients and to evaluate the relationship between the content of adipokines and myokines and the content of tryptophan metabolism metabolites of bacterial and non-bacterial origin in the blood serum of patients with MSO and MNSO.

Material and methods. 266 patients were examined, including 138 healthy non-obese volunteers and 128 obese patients, of whom two subgroups were formed: 30 patients with MHO and 41 patients with MUHO. Metabolite concentrations in blood and feces were determined using high performance liquid chromatography. Quantitative analysis of adipokines and myokines was performed by multiplex enzyme immunoassay. The content of metabolites of tryptophan metabolism in blood serum was assessed by high performance liquid chromatography with mass spectrometric detection.

Results. For patients with obesity, an increase in the serum concentration of kynurenine, kynurenic and quinoline acids, indole-3-lactate, indole-3-butyrate and indole-3-acetate was established. Patients with MHO and MUHO were statistically significantly different only in terms of serum tryptamine concentration. Regardless of the presence/absence of metabolic disorders in obese patients, it has been established that xanthurenic and quinoline acids are interrelated with the concentration of myostatin in the blood serum. At the same time, for patients with MHO, it was shown that the serum concentration of the dominant catabolite of the indole pathway, indole-3-acetate, is interconnected with the content of insulin and leptin in the blood serum. Whereas in patients with MUHO, leptinemia is consistent with a high concentration of anthranilic acid in the blood serum, and hyperinsulinemia, on the contrary, is associated with a low concentration of indole-3-propionate in the blood serum.

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作者简介

O. Shatova

Pirogov Russian National Research Medical University

编辑信件的主要联系方式.
Email: shatova.op@gmail.com
ORCID iD: 0000-0003-4265-1293

Ph.D. (Med.), Associate Professor of the Department of Biochemistry and Molecular Biology

俄罗斯联邦, Moscow

S. Appolonova

Institute of Translational Medicine and Biotechnology, First Moscow State Medical University (Sechenov University)

Email: shatova.op@gmail.com
ORCID iD: 0000-0002-9032-1558

Ph.D. (Chem.), Head of the Center for Biopharmaceutical Analysis and Metabolomic Research

俄罗斯联邦, Moscow

S. Roumiantsev

Pirogov Russian National Research Medical University

Email: shatova.op@gmail.com
ORCID iD: 0000-0002-7418-0222

Dr.Sc. (Med.), Professor, Corresponding Member of the Russian Academy of Sciences, Head of the Department of Oncology, Hematology and Radiation Therapy, Faculty of Pediatrics

俄罗斯联邦, Moscow

A. Shestopalov

Pirogov Russian National Research Medical University

Email: al-shest@yandex.ru
ORCID iD: 0000-0002-1428-7706

Dr.Sc. (Med.), Professor, Head of the Department of Biochemistry and Molecular Biology

俄罗斯联邦, Moscow

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