A new method of tincture preparation in the base of saposhnikovia divaricata roots – the source of chromones, its standatization and determination of antiradical activity

Relevance. Saposhnikovia divaricata (Turcz.) Schischk. is a rich source of chromones. Dry and liquid extracts, preparations of individual substances, as well as cosmetics for problem skin are produced from the roots of S. divaricata. However, there are no data of the tincture preparation in the base of on the S. divaricata despite their ease of administration.

The aim of the work is to develop a new method of tincture preparation in the base of S. divaricata roots, its standardization and determination of antiradical activity.

Material and methods. To obtain the tincture, we used the raw material S. divaricata roots produced by the "Daurskaya zagotovitel'naya kompaniya" in 2019. The choice of the optimal method for the tincture preparation was carried out using various periodic and intensive extraction methods. Ethyl alcohol was used as an extractant in the concentration range from 40 to 70%. For maximum extraction of chromones, the degree of grinding of raw materials was selected. Determination of the quantitative content of chromones was carried out by HPLC-UV using solutions of standard samples of prim-O-glucosylcymifugin, cymifugin and 4'-O-β-D-glucosyl-5-O-methylvissaminol. The antiradical activity of the tincture was evaluated using the DPPH test.

Results. The optimal conditions for the tincture preparation from the roots of S. divaricata splayed by the output of active substances - prim-O-glucosylcymifugin, cymifugin and 4'-O-β-D-glucosyl-5-O-methylvissaminol were selected. The optimal extraction parameters have been established: extractant (50% ethyl alcohol), degree of grinding (raw material, passing through a sieve with a diameter of 0.5 mm).

Conclusions. A method of tincture preparation in the base of S. divaricate roots has been developed, and the optimal conditions for its production have been selected. Extraction on the Soxhlet apparatus makes it possible to isolate twice as many chromones as compared to other extraction and circulation methods for tincture preparation. The best extractant of 50% ethyl alcohol. The optimal size of root grinding is 0.5 mm. The antiradical activity of the tincture was determined by the DPPH test.