Antioxidant activity of 2-aryl-7,7-dimethyl-5-oxo-5,6,7,8-tetrahydroquinoline-4-carboxylic acids

Relevance. Oxidative stress is an imbalance between the production of reactive oxygen species and the ability of the endogenous antioxidant system, and is also part of the pathogenesis of a number of diseases. Recently, antioxidants have been included in the complex treatment of many pathological conditions, so the search for compounds with an antioxidant effect is relevant.

Aim. Study of the antioxidant activity of 2-aryl-7,7-dimethyl-5-oxo-5,6,7,8-tetrahydroquinoline-4-carboxylic acids in a model of oxidative stress induced by hydrogen peroxide in a cell culture of E. coli strain "Ecolume."

Material and methods. 2-Aryl-7,7-dimethyl-5-oxo-5,6,7,8-tetrahydroquinoline-4-carboxylic acids were obtained as a result of the interaction of 4-aroyl-2,4-dioxobutane acids with 3-amino-5,5-dimethylcyclohex-2-enone. The evaluation of the antioxidant activity of the synthesized compounds was carried out by an indirect method of assessing changes in the fluorescence of E. coli cell culture strain "Ecolume" using a model of oxidative stress induced by hydrogen peroxide.

Results. Among 2-aryl-7,7-dimethyl-5-oxo-5,6,7,8-tetrahydroquinoline-4-carboxylic acids, compounds with antioxidant activity exceeding the reference standard – trolox were found.

Conclusions. Investigation of the antioxidant activity of 9 compounds of the 2-aryl-7,7-dimethyl-5-oxo-5,6,7,8-tetrahydroquinoline-4-carboxylic acid series using E. coli cell culture as a biosensor.