Hepatoprotective effect of golden volodushka (Bupleurum Aureum L.) Herb extract of dry in experimental toxic hepatitis in rats. Problems of biological, medical and pharmaceutical chemistry

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Introduction. The search for new medicines, including those of plant origin, for the treatment of diseases of the hepatobiliary system is relevant and timely. A promising source for the development of hepatoprotective drugs is golden volodushka (Bupleurum aureum L.). To date, a large evidence base has been accumulated for the biochemical study of this plant, its pharmacological activity has been scientifically proven. Good stocks of golden volodushka in the wild, the simplicity of its harvesting and the possibility of cultivation were noted. The VILAR State Medical University has developed a method for obtaining volodushka golden herb extract of dry.

Aim. The study of the pharmacotherapeutic efficacy of volodushka golden (Bupleurum aureum L.) herb extract of dry in case of liver damage under experimental conditions.

Material and methods. In the studies, white non-linear male rats with a body weight of 180-200 g were used. Volodushka extract was studied at doses of 50 and 100 mg/kg. The comparison drug was silimar at a dose of 100 mg/kg. Experimental CCl4 – induced hepatitis in rats was caused by a single subcutaneous injection of 50% carbon tetrachloride oil solution one hour after the last administration of the studied extract and the comparison drug. After 48 hours, blood was taken from the tail vein of rats, serum was obtained and its biochemical analysis was carried out. Next, the rats euthanized in a CO2 chamber and the liver extracted for pathophysiological evaluation. Tetracycline hepatitis was caused by intragastric administration of tetracycline to animals at a dose of 500 mg/kg. Then, after 48 hours, blood was taken from the animals, serum was obtained and its biochemical analysis was carried out.

Results. Volodushka golden grass dry extract on a model of carbon tetrachloride hepatitis exhibits a hepatoprotective effect, which is confirmed by a comparative pathophysiological study and a study of the activity of enzyme markers of the morphofunctional state of the liver in rat blood serum. In the tetracycline hepatitis model, the studied extract also has a hepatoprotective effect, which is confirmed by biochemical studies.

Conclusions. Studies of volodushka extract in conditions of experimental liver lesions have shown that the studied extract in experimental therapeutic doses has a pronounced hepatoprotective effect and is promising for the creation of a herbal medicine for the prevention and treatment of diseases of the digestive system.

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作者简介

E. Kurmanova

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

编辑信件的主要联系方式.
Email: kurmanova1968@yandex.ru
ORCID iD: 0000-0002-9243-5268

Ph.D. (Biol.), Research Scientist at the Department of Experimental Pharmacology

俄罗斯联邦, Grina str. 7, 117216, Moscow

E. Ferubko

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Email: eferubko@yandex.ru
ORCID iD: 0000-0003-1949-2597

Dr.Sc. (Med.), Head of the Department of Experimental Pharmacology

俄罗斯联邦, Grina str. 7, 117216, Moscow

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2. Fig. 1. Morphofunctional structure of the liver of rats: a –  the intact group; b –  the control group. Staining with hematoxylin and eosin, magnification x 100

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3. Fig. 2. Morphofunctional structure of the liver of rats treated with volodushka extract at a dose: a – 50 mg/kg; b – 100 mg/kg. Staining with hematoxylin and eosin, magnification x 100

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4. Figure 3. Morphofunctional structure of the liver of rats treated with silimar at a dose of 100 mg/kg. Staining with hematoxylin and eosin, magnification x 100

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