The amino acid composition of Haplophyllum Dauricum (l.) G. Don

Introduction. Haplophyllum dauricum, a well-known source of lignans, is widely used in traditional medicine. Additionally, data on its chemical composition includes information on alkaloids, terpenoids, coumarins, flavonoids, and the macro- and microelement content. However, no information is available regarding the amino acid profile of this plant.

The aim of the study is to determine qualitative and quantitative composition of amino acids in herbs and roots of H. dauricum.

Material and methods. The objects of the study were the herbs and roots of H. dauricum native to the Republic of Buryatia. Samples were collected during the flowering and fruiting periods in 2022 and 2023. The amino acid composition and content were determined using the capillary electrophoresis system "Kapel-105". Statistical analysis of the obtained data was performed using the principal component analysis (PCA) method with the Sirius version 6.0 (Pattern Recognition Systems, a/s, Norway).

Results. Twenty amino acids were found in the herbs and roots parts of H. dauricum, with nonessential amino acids predominating (up to 80.81% of the total). Glutamic acid, glutamine, aspartic acid, and asparagine have the highest content, while proline, arginine, methionine, and serine also have high levels. A change in the quantity of diaminocarboxylic and heterocyclic amino acids was observed in response to abiotic stress.

Conclusions. This is the first study of the qualitative composition and quantitative content of amino acids in H. dauricum. Eight essential amino acids were identified, with glutamic acid, glutamine, aspartic acid, and asparagine being dominant. The data suggest potential for further research on this species and its potential as a source of valuable bioactive compounds.