Search for preservation solutions of low toxicity for conservation wet anatomical preparations

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Introduction. Anatomical preparations are not only important auxiliary material in training highly qualified specialists in the natural sciences but also form the basis for scientific research. Most demonstration and educational preparations are wet, that is, they are conserved in preservative liquids and require constant monitoring and periodic restoration. Formalin, despite its well-known carcinogenic, mutagenic and toxic properties, remains the most commonly used preservative solution worldwide. Thus, there has long been a need to search for alternative solutions that also have antiseptic, fixing and preserving properties, but are safe for human health and the environment.

The aim of the study is to conduct a literature review to search for promising options for preservative solutions that do not contain formalin; consider their safety and effect on fixed tissues, study the positive and negative properties.

Material and methods. The review includes data from studies studying various methods of fixing pathological material for the manufacture of wet anatomical preparations. The sources for the review were the PubMed, Google Scholar, ResearchGate, eLibrary.ru, CyberLeninka databases.

Results. This review examines monocomponent preservative solutions based on glyoxal, sodium benzoate, sodium azide, polyhexamethylene guanidine hydrochloride and saturated salt solutions and conducts a comparative analysis of them.

Conclusion. Currently developed alternative preservative solutions have a number of significant drawbacks, which prevents them from becoming a full-fledged replacement for formalin. Further search and improvement of methods for preserving pathological preparations should make it possible in the future to abandon the use of preservative solutions that contain toxic substances that pose a danger to human health and the environment.

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作者简介

T. Kuznetsova

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

编辑信件的主要联系方式.
Email: tatyana142@yandex.ru
ORCID iD: 0009-0005-4486-9845
SPIN 代码: 2789-8807

Junior Research Scientist, Research and Educational Methodological Center of Biomedical Technologies 

俄罗斯联邦, 7 Grin str., Moscow, 117216

V. Krasnov

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Email: v.v.krasnov@mail.ru
ORCID iD: 0000-0002-7415-4817
SPIN 代码: 1393-8273

Dr.Sc. (Biol.), Head of the Department of Medical and Biological Problems, Head of the Research and Educational Methodological Center of Biomedical Technologies 

俄罗斯联邦, 7 Grin str., Moscow, 117216

Yu. Gribunov

All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Email: gup44@mail.ru
ORCID iD: 0009-0006-0676-5866
SPIN 代码: 5863-0064

Dr.Sc. (Med.), Professor, Chief Research Scientist, Research and Educational Methodological Center of Biomedical Technologies

俄罗斯联邦, 7 Grin str., Moscow, 117216

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2. Formaldehyde

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3. Glyoxal

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4. Sodium benzoate

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5. Sodium azide

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6. Polyhexamethylene guanidine hydrochloride

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