Changes in protein expression of rat astrocytes co-cultured with C6 glioma cells


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Abstract

Introduction. Based on current literature data, it is possible to assume that when exposed to glioma cells astrocytes, may undergo a reactive transformation. These glioma-conditioned astrocytes might create a permissive environment for tumorigenesis. The aim of the study. To investigate the effects of glioma cells on astrocytes in co-culture. Methods. In terms of the current work, we conducted a pairwise comparison of protein levels between C6 glioma cells, native rat astrocytes, and glioma-conditioned rat astrocytes. The samples were prepared and analyzed with liquid chromatography-high-resolution mass spectrometry. Results. The analysis showed a significant difference in 162 proteins between glioma cells and native astrocytes, in 141 proteins between glioma cells and glioma-conditioned astrocytes and 70 proteins between glioma-conditioned and native astrocytes. Conclusion. The differences in protein levels between native and glioma-conditioned astrocytes show a high correlation with differences between glioma cells and native astrocytes.

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About the authors

A. S Silantyev

National Scientific Research Center on Addictions a branch of the V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology

Maly Mogiltsevsky by-streeT., 3, Moscow, 119002, Russian Federation

I. V Chekhonin

V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology

Kropotkinskiy by-streeT., 23, Moscow, 119034, Russian Federation

A. A Chernysheva

V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology

Email: aachernysheva512@gmail.com
Kropotkinskiy by-streeT., 23, Moscow, 119034, Russian Federation

O. I Gurina

V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology

Kropotkinskiy by-streeT., 23, Moscow, 119034, Russian Federation

S. A Pavlova

Institute of Gene Biology Russian Academy of Sciences

Vavilova StreeT., 34/5, Moscow, 119334, Russian Federation

G. V Pavlova

Institute of Gene Biology Russian Academy of Sciences; Sechenov First Moscow State Medical University; N.N. Burdenko National Medical Research Center of Neurosurgery

Institute of Molecular Medicine Vavilova StreeT., 34/5, Moscow, 119334, Russian Federation; Trubetskaya StreeT., 8, Moscow, 119991, Russian Federation; 4 Tverskaya-Yamskaya str., 16, Moscow, 125047, Russian Federation

T. A Savelieva

Prokhorov General Physics Institute; National Research Nuclear University

Moscow Engineering Physics Institute Vavilova str., 38, Moscow, 119991, Russian Federation; Kashirskoe Highway, 31, Moscow, 115409, Russian Federation

V. B Loshhenov

Prokhorov General Physics Institute; National Research Nuclear University

Moscow Engineering Physics Institute Vavilova str., 38, Moscow, 119991, Russian Federation; Kashirskoe Highway, 31, Moscow, 115409, Russian Federation

V. P Chekhonin

V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology; Pirogov Russian National Research Medical University

Kropotkinskiy by-streeT., 23, Moscow, 119034, Russian Federation; Ostrovitianova str., 1, Moscow, 117997, Russian Federation

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