The role of markers of intercellular interaction in the development of atopic dermatitis

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Abstract

Introduction. Violation of the skin barrier can be considered as an initial stage in the development of atopic dermatitis, leading to further skin inflammation [2]. Transmembrane proteins such as claudin-1, claudin-7, occludin and E-cadherin are known to be the main components of epidermal tight junctions (TJ) [15]. Objective. The aim of the study was to research the pathogenesis of atopic dermatitis in cell culture and to assess the effect of placental hydrolysate on blood pressure. Methods. The studies were carried out on the cell culture of normal fibroblasts and the cell culture of atopic dermatitis. An immunocytochemical study was conducted to evaluate intercellular communications. Results. When the placenta hydrolysate was administered, the expression levels of occludin, claudin-1, claudin-7 and E-cadherin in blood in group IV were significantly different from those of group II. Thus, all the studied proteins contribute significantly to the development of the pathogenesis of atopic dermatitis. Conclusion. These results indicate that the studied markers play an important role in the pathogenesis of atopic dermatitis atopic dermatitis The work carried out allows us to understand in more detail the pathological processes occurring in this disease and prescribe drugs containing placental hydrolysate to restore normal epithelial differentiation.

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About the authors

Ekaterina Leonidovna Iskra

St. Petersburg State Pediatric Medical University Ministry of Health of the Russian Federation

Author for correspondence.
Email: doc@mail.ru
postgraduate student of the Department of Pathological Anatomy with the course of Forensic Medicine 194100, Russian Federation, St. Petersburg, Litovskaya Street, 2

Alexander Sergeevich Iskra

St. Petersburg State Pediatric Medical University Ministry of Health of the Russian Federation

Email: neonatol@list.ru
postgraduate student of the Department of Rehabilitation of FP and DPO 194100, Russian Federation, St. Petersburg, Litovskaya Street, 2

Viktoriya Olegovna Polyakova

St. Petersburg Research Institute of Phthisiopulmonology Ministry of Health of the Russian Federation

Email: vopol@yandex.ru
Deputy Director for Scientific Work; Doctor of Biological Sciences, Professor 191036, Russian Federation, St. Petersburg, Ligovsky Prospekt, 2-4

Ruslan Abdullaevich Nasyrov

St. Petersburg State Pediatric Medical University Ministry of Health of the Russian Federation

Email: rrmd99@mail.ru
Vice-Rector for Research, Head of the Department of Pathological Anatomy with the Course of Forensic Medicine; Doctor of Medical Sciences, Professor. 194100, Russian Federation, St. Petersburg, Litovskaya Street, 2

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Supplementary files

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2. Fig. 1. The level of occludin expression in cell cultures Note. * – significant difference from group I (p<0,05).

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3. Fig. 2. Expression level of claudin-1 in cell cultures Note. * – significant difference from group I (p<0,05).

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4. Fig. 3. Expression level of claudin-7 in cell cultures

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5. Fig. 4. The expression level of E-cadherin in cell cultures

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