Stimulating effect of encoded amino acids combinations on cellular proliferation in organotypic culture of tissue
- Authors: Chalisova N.I.1,2, Ryzhak G.A.2
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Affiliations:
- Pavlov Institute of Physiology, Russian Academy of Sciences
- St. Petersburg Institute of Bioregulation and Gerontology
- Issue: Vol 22, No 3 (2024)
- Pages: 53-56
- Section: Reviews
- URL: https://journals.eco-vector.com/1728-2918/article/view/633670
- DOI: https://doi.org/10.29296/24999490-2024-03-08
- ID: 633670
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Abstract
Introduction. The study of mechanisms of multicellular system regulation and of cellular proliferation balanced status in the organism tissues is one of the actual task of modern biology and medicine.
Purpose of study. Effect of encoded amino acids combinations on the cellular proliferation in tissues of different genesis.
Methods. Organotypic tissue culture of 5-month rat cerebral cortex (ectodermal genesis), myocardium (mezodermal genesis), pancreas (entodermal genesis) was used for quick screening of biologically activity of amino acids.
Results. The stimulating cellular proliferation effect of encoded amino acids combinations on tissues was established. The square index (SI) of explants was increased statistically reliable on 38–70%, as compared to the control.
Conclusion. The data obtained produce the base for working of preparations for treatment of patients with pathology in cerebral cortex, myocardium and pancreas tissues.
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About the authors
Natalia I. Chalisova
Pavlov Institute of Physiology, Russian Academy of Sciences; St. Petersburg Institute of Bioregulation and Gerontology
Author for correspondence.
Email: ni_chalisova@mail.ru
ORCID iD: 0000-0002-2371-0043
Expert scientific worker, Expert scientific worker of the laboratory of Oncogerontology of Biogerontology department, Doctor of biologic sciences, professor
Russian Federation, emb. Makarova, 6, St. Petersburg, 199034; Dynamo Ave., 3, St. Petersburg, 197110
Galina A. Ryzhak
St. Petersburg Institute of Bioregulation and Gerontology
Email: galina@gerontology.ru
ORCID iD: 0000-0003-2536-1438
associate Director of Institute, Doctor of medical sciences, рrofessor
Russian Federation, Dynamo Ave., 3, St. Petersburg, 197110References
- Aftabuddin Md., Kundu, S. Hydrophobic,hydrophilic, and charged amino acid networks within protein. Biophysical J. 2007; 93: 225–31. https://www.doi.org/10.1529/biophysj.106.098004
- Чалисова Н.И., Концевая Е.А., Войцеховская М.А. Регуляторное влияние кодируемых аминокислот на основные клеточные процессы у молодых и старых животных. Успехи геронтологии. 2011; 2: 189–97. [Chalisova N.I., Koncevaya E.A., Voicekhovskaya M.A. Regulating effect of coded amino acids on main cellular processes in young and old animals. Advance in gerontology. 2011; 2: 189–97 (In Russian)].
- Хавинсон В.Х., Чалисова Н.И., Линькова Н.С., Халимов Р.И., Ничик Т.Е. Зависимость тканеспецифического действияпептидов от их количественного аминокислотного состава.Фундаментальные исследования. 2015; 2: 497–503. [Khavinson V.H., Chalisova N.I., Linkova N.S.,Khalimov R.I., Nichik T.E. The dependence of tissue-specific peptides activity on the number of amino acids in the peptides.Fundamental Researches. 2015; 2: 497–503 (In Russian)].
- Fedoreyeva L.I., Kireev I.I., Khavinson V.Kh.,Vanyushin B.F. (2011) Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry. 2011; 76 (11): 1210–9.
- Vanyushin B.F., Khavinson V.Kh. Short Biologically Active Peptides as Epigenetic Modulators of Gene Activity. Epigenetics – A Different Way of Looking at Genetics. W. Doerfler, P. Böhm (eds.). Springer International Publishing Switzerland. 2016; 69–90.
- Anisimov V.N., Khavinson V.Kh. Peptide bioregulation of aging: results and prospects. Biogerontology. 2010; 11: 139–49. doi: 10.1007/s10522-009-9249-8.
- Рыжак А.П., Чалисова Н.И., Линькова Н.С. Влияние полипептидов на регенерацию клеток в культуре разных тканей молодых и старых крыс. Успехи геронтологии. 2015; 28 (1): 97–103. [Rizhak G.A., Chalisova N.I., Linkova N.S. Polypeptide effect on cellular regeneration in different tissue culture of young and old rats. Advances in Gerontology. 2015; 28 (1): 97–103 (In Russian)].
- Журкович И.К., Ковров Н.Г., Рыжак Г.А., Миронова Е.С., Хавинсон В.Х. Идентификация коротких пептидов в составе полипептидных комплексов, выделенных из органов животных. Успехи современной биологии. 2020; 140 (2): 140–8. doi: 10.31857/S004213242002012X. [Zhurkovich I.K., Kovrov N.G., Rizhak G.A., Mironova E.S., Khavinson V. Rh. Identification of short peptides in the composition of polypeptide complexes from animal organs. Advances of modern biology. 2020; 140 (2): 140–8. DOI: 10.31857/ S004213242002012X (In Russian)].
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