Significance of altered neutrophil extracellular traps formation during the development of pregnancy complications among patients with gestational diabetes mellitus

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Introduction. The interest in neutrophil extracellular traps (NETs) in various conditions, including pregnancy complications was increased over the last decade. Accompanied by gestational diabetes mellitus (GDM) hyperglycemia contributes to neutrophil’s activation and the formation of NETs.

The aim of study. In this study, we assess NETosis and placental function parameters of women who received diet only or insulin for treatment of gestational diabetes (GDMD/GDMI).

Methods. PR3 circulating levels, associated with NETs formation and placental expression were analyzed by ELISA and immunohistochemistry respectively in GDMD (n=35), GDMI (n=30) and healthy pregnant women (n=30). Clinical data, placental alpha microglobulin-1 (PAMG), placental lactogen and trophoblast glycoprotein (TGB) immunohistochemistry expression were characterized in 30 placentas of GDMD, 20 – GDMI and 25 uncomplicated pregnancies.

Results. PR3 placental expression was increased in complicated pregnancies (GDMD – 60,94 vs 31,88, p<0,001, GDMI – 64,42 vs 31,88, р=0,009) with no statistical differences between GDMD and GDMI. Though placental protein expression indicated potential ischemia among GDMI patients, newborn’s condition was the same like healthy mother’s group. Placental protein expression and clinical data between GDMD and uncomplicated pregnancy women were similar.

Conclusion. The study proposes that NETosis in GDM patients in adequate therapy condition, perhaps don’t stimulate pregnancy complications, in contrast – presents consequences of preexisting placental damage.

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作者简介

Marina Shamarakova

S.S. Yudin City Clinical Hospital

编辑信件的主要联系方式.
Email: mshamarakova@yandex.ru
ORCID iD: 0000-0002-0972-4350

Candidate of Medical Sciences, Pathologist of pathology department

俄罗斯联邦, Kolomenskiy passage, 4, Moscow, 115446

Ksenia Artem`eva

Avtsyn Research Institute of Human Morphology of FSBSI “Petrovsky National Research Center of Surgery”

Email: artemjeva_ksenia@mail.ru
ORCID iD: 0000-0002-1014-752X

Candidate of Medical Sciences, Senior researcher of the reproduction pathology laboratory

俄罗斯联邦, Abrikosovsky lane, 2, Moscow, 119991

Natalia Nizyaeva

Avtsyn Research Institute of Human Morphology of FSBSI “Petrovsky National Research Center of Surgery”

Email: nizyaeva@gmail.com
ORCID iD: 0000-0001-5592-5690

Doctor of Medical Sciences, Head of the reproduction pathology laboratory

俄罗斯联邦, Abrikosovsky lane, 2, Moscow, 119991

Georgy Zayratyants

S.S. Yudin City Clinical Hospital

Email: goshaz@mail.ru
ORCID iD: 0000-0002-9265-5017

Candidate of Medical Sciences, Head of Pathology Department

俄罗斯联邦, Kolomenskiy passage, 4, Moscow, 115446

Irina Stepanova

Avtsyn Research Institute of Human Morphology of FSBSI “Petrovsky National Research Center of Surgery”

Email: i-ste@yandex.ru
ORCID iD: 0000-0002-5513-217X

Researcher of the reproduction pathology laboratory

俄罗斯联邦, Abrikosovsky lane, 2, Moscow, 119991

Alexandr Stepanov

Avtsyn Research Institute of Human Morphology of FSBSI “Petrovsky National Research Center of Surgery”

Email: 9163407056@mail.ru
ORCID iD: 0000-0002-5036-1387

Researcher of the reproduction pathology laboratory

俄罗斯联邦, Abrikosovsky lane, 2, Moscow, 119991

Alina Akhmetshina

Avtsyn Research Institute of Human Morphology of FSBSI “Petrovsky National Research Center of Surgery”

Email: malina.alina2001@mail.ru
ORCID iD: 0009-0005-6366-6031

Researcher of the reproduction pathology laboratory

俄罗斯联邦, Abrikosovsky lane, 2, Moscow, 119991

Yulia Dobrokhotova

FSAEI HE “N.I.Pirogov Russian National Research Medical University” of the Ministry of Health of the Russian Federation

Email: pr.dobrohotova@mail.ru
ORCID iD: 0000-0001-6571-3448

Doctor of Medical Sciences, Professor, Head of the Department of Obstetrics and Gynecology, Faculty of Medicine

俄罗斯联邦, Ostrovityanova str., 1, Moscow, 117513

Liudmila Mikhaleva

Avtsyn Research Institute of Human Morphology of FSBSI “Petrovsky National Research Center of Surgery”

Email: mikhalevalm@yandex.ru
ORCID iD: 0000-0003-2052-914X

Doctor of Medical Sciences, Professor, Corresponding Member of RAS, Director, Head of the Laboratory of Clinical Morphology

俄罗斯联邦, Abrikosovsky lane, 2, Moscow, 119991

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2. Fig. 1. The meanings of blood tests results between patients with gestational diabetes mellitus and healthy pregnant women. There were no significant differences between groups

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3. Fig. 2. Immunohistochemical staining of placental specimens of gestational diabetes mellitus and healthy pregnant for PAMG (а–в), PL (г–е), TBG (ж–и) × 200) Note: а, г, ж – GDMD; б, д, з – GDMI; в, е, и – physiological pregnancy.

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