The influence of L-amino acids on the viability of neuroendocrine PC 12 cells line


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Introduction. It was shown, that L-amino acids can take part in regulation of the main physiological processes. Various combination of L-amino acids takin into account of its biological activity can apply for peptide drugs synthesis for target therapy of social significant diseases. The aim. Estimation of the influence of 20L-amino acids on the viability of PC12 cell line. Methods. The investigation was based on MTT test applying for viability estimation of PC12 cell line. Results. Methionine, arginine, asparagine, glutamic acid and histidine increased the viability of PC12 cell line on 54, 56, 51, 63 and 27% accordingly. Conclusion. The molecular mechanism of histidine and arginine activity can be connected with epigenetic genes regulation of cell metabolism. Previously it was shown that 5 amino acids stimulated nerve and pancreatic tissues grown in various aged animals. It can be constructed short peptides on the base of methionine, arginine, asparagine, glutamic acid and histidine. These peptides can be perspectives substances for prevention and therapy age-related neuroendocrine pathology.

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作者简介

Natalia Linkova

Saint Petersburg Institute of Bioregulation and Gerontology; Pavlov Institute of Physiology, Russian Academy of Sciences; Academy of postgraduate education under FSBU FSCC of FMBA of Russia

Email: miayy@yandex.ru
doctor of biological sciences, associate professor, the head of the laboratory of Molecular mechanisms of aging of Biogerontology department; geriatrics and anti-age medicine; senior researcher of the laboratory “Problems of aging”

Natalia Chalisova

Saint Petersburg Institute of Bioregulation and Gerontology; Belgorod State University

Email: ni_chalisova@mail.ru
Doctor of biological sciences, рrofessor, senior researcher; senior researcher of the laboratory of Oncogerontology of Biogerontology department

Galina Ryzhak

Saint Petersburg Institute of Bioregulation and Gerontology

Email: galina@gerontology.ru
Doctor of medical sciences, рrofessor, associate Director

Ekaterina Gutop

Saint Petersburg Institute of Bioregulation and Gerontology

Email: mgutop@mail.ru
MD, PhD, senior researcher of the laboratory of Molecular mechanisms of aging of Biogerontology department

Oleg Ivko

Saint Petersburg Institute of Bioregulation and Gerontology

Email: oleg.ivko@gmail.com
MD, PhD, senior researcher of the laboratory of Molecular mechanisms of aging of Biogerontology department

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