The influence of L-amino acids on the viability of neuroendocrine PC 12 cells line


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Introduction. It was shown, that L-amino acids can take part in regulation of the main physiological processes. Various combination of L-amino acids takin into account of its biological activity can apply for peptide drugs synthesis for target therapy of social significant diseases. The aim. Estimation of the influence of 20L-amino acids on the viability of PC12 cell line. Methods. The investigation was based on MTT test applying for viability estimation of PC12 cell line. Results. Methionine, arginine, asparagine, glutamic acid and histidine increased the viability of PC12 cell line on 54, 56, 51, 63 and 27% accordingly. Conclusion. The molecular mechanism of histidine and arginine activity can be connected with epigenetic genes regulation of cell metabolism. Previously it was shown that 5 amino acids stimulated nerve and pancreatic tissues grown in various aged animals. It can be constructed short peptides on the base of methionine, arginine, asparagine, glutamic acid and histidine. These peptides can be perspectives substances for prevention and therapy age-related neuroendocrine pathology.

Full Text

Restricted Access

About the authors

Natalia Sergeevna Linkova

Saint Petersburg Institute of Bioregulation and Gerontology; Pavlov Institute of Physiology, Russian Academy of Sciences; Academy of postgraduate education under FSBU FSCC of FMBA of Russia

Email: miayy@yandex.ru
doctor of biological sciences, associate professor, the head of the laboratory of Molecular mechanisms of aging of Biogerontology department; geriatrics and anti-age medicine; senior researcher of the laboratory “Problems of aging”

Natalia Iosifovna Chalisova

Saint Petersburg Institute of Bioregulation and Gerontology; Belgorod State University

Email: ni_chalisova@mail.ru
Doctor of biological sciences, рrofessor, senior researcher; senior researcher of the laboratory of Oncogerontology of Biogerontology department

Galina Anatol’evna Ryzhak

Saint Petersburg Institute of Bioregulation and Gerontology

Email: galina@gerontology.ru
Doctor of medical sciences, рrofessor, associate Director

Ekaterina Olegovna Gutop

Saint Petersburg Institute of Bioregulation and Gerontology

Email: mgutop@mail.ru
MD, PhD, senior researcher of the laboratory of Molecular mechanisms of aging of Biogerontology department

Oleg Michailovich Ivko

Saint Petersburg Institute of Bioregulation and Gerontology

Email: oleg.ivko@gmail.com
MD, PhD, senior researcher of the laboratory of Molecular mechanisms of aging of Biogerontology department

References

  1. Khavinson V., Linkova N., Diatlova A., Trofimova S. Peptide regulation of cell differentiation. Stem Cell Reviews and Reports. 2020; 16: 118-25. https://doi.org/10.1007/s12015-019-09938-8.
  2. Khavinson V., Diomede F., Mironova E., Linkova N., Trofimova S., Trubiani O., Caputi S., Sinjari B. AEDG Peptide (Epitalon) stimulates gene expression and protein synthesis during neurogenesis: possible epigenetic mechanism. Molecules. 2020; 25 (3): 1-17. https://doi.org/10.3390/molecules25030609.
  3. Khavinson V., Linkova N., Kozhevnikova E., Trofimova S. EDR peptide: possible mechanism of gene expression and protein synthesis regulation involved in the pathogenesis of Alzheimer's disease. Molecules. Special Issue «Peptide Therapeutics 2.0». 2021; 26 (1): 1-16. https://doi.org/10.3390/molecules26010159.
  4. Khavinson V., Linkova N., Dyatlova A., Kuznik B., Umnov R. Peptides: prospects for use in the treatment of COVID-19. Molecules. Special Issue «Peptide Therapeutics 2.0». 2020; 25 (10). 4389. https://doi.org/10.3390/molecules25194389.
  5. Bonfili L., Cecarini V., Cuccioloni M., Angeletti M., Flati V., Corsetti G., Pasini E., Dioguardi F.S., Eleuteri A.M. Essential amino acid mixtures drive cancer cells to apoptosis through proteasome inhibition and autophagy activation. FEBS. 2017; 284 (11): 1726-37. https://doi.org/10.1111/febs.14081.
  6. Kimura M., Ogihara M. Effects of branchedchain amino acids on DNA synthesis and proliferation in primary cultures of adult rat hepatocytes. Eur. J. Pharmacol. 2005; 510 (3): 167-80. https://doi.org/10.1016/j.ejphar.2005.01.011.
  7. Bolam J.P., Ellender T.J. Histamine and the striatum. Neuropharmacology. 2016; 106: 74-84. htt-ps://doi.org/10.1016/j.neuropharm.2015.08.013
  8. Cruzat V., Macedo M., Rogero K., Noel Keane N., Curi R., Newsholme P. Glutamine: metabolism and immune function, supplementation and clinical translation. Nutrients. 2018; 10 (1): 1564. https://doi.org/10.3390/nu10111564
  9. Chalisova N.I., Koncevaya E.A., Linkova N.S., Pronyaeva V.E., Cherviakova N.A., Umnov R.S., Benberin V.V., Khavinson VKh. Biological activity of amino acids in organotypic tissue cultures. Bull. Exp. Biol. Med. 2013; 155 (4): 581-5. https://doi.org/10.1007/s10517-013-2200-7
  10. Chalisova N.I., Kontzevaya E.A., Voitzekhovskaya M.A., Komashnya A.V Regulating effect of coded amino acids on basic cellular processes of young and old animals. Advances in Gerontology. 2011; 24 (2): 189-97
  11. Чалисова Н.И., Камышев Н.Г., Лопатина Н.Г, Концевая Е.А., Уртьева С.А., Уртьева ТА. Влияние кодируемых аминокислот на ассоциативное обучение медоносной пчелы. Apis Mellifera. Журнал эволюционной биохимии и физиологии. 2011; 46 (6): 516-8
  12. Хавинсон В.Х., Чалисова Н.И., Линькова Н.С., Халимов Р.И., Ничик ТЕ. Зависимость тканеспецифического действия пептидов от их количественного аминокислотного состава. Фундаментальные исследования. 2015; 2: 497-503.
  13. Koncevaya E.A., Linkova N.S., Chalisova N.I., Dudkov A.V, Sinyachkin D.A. Effect of amino acids on expression of signal molecules in organotypic culture of the spleen. Bull. Exp. Biol. Med. 2012; 153 (4): 573-6. https://doi.org/10.1007/s10517-012-1769-6.
  14. Wu C., Zhao W., Yu J., Li S., Lin L., Chen X. Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells. Sci Rep. 2018; 8 (1): 574. https://doi.org/10.1038/s41598-017-18935-1.
  15. Tian J.S., Liu S.B., He X.Y., Xiang H., Chen J.L., Gao Y, Zhou Y.Z., Qin X.M. Metabolomics studies on corticosterone-induced PC12 cells: A strategy for evaluating an in vitro depression model and revealing the metabolic regulation mechanism. Neurotoxicol. Teratol. 2018; 69: 27-38. https://doi.org/10.1016/j.ntt.2018.07.002.
  16. Li L., Sun H.Y, Liu W., Zhao H.Y, Shao M.L. Silymarin protects against acrylamide-induced neurotoxicity via Nrf2 signalling in PC12 cells. Food Chem. Toxicol. 2017; 102: 93-101. https://doi.org/10.1016/j.fct.2017.01.021.
  17. Hoffman M.M., Khrapov M.A., Cox J.C., Yao J., Tong L., Ellington A.D. AANT: the Amino Acid-Nucleotide Interaction Database. Nucleic Acids Res. 2004; 32: 174-81. https://doi.org/10.1093/nar/gkh128.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies