ANALYSIS OF METHYLATION STATUS ANALYSIS OF RASSF1A, BRCA1, HIN1IN BREAST AND OVARIAN CANCER CELL LINES
- Authors: Potapova AA1, Ostrovskiy O.V.1, Dudchenko GP1, Potapova AA1, Ostrovskiy OV1, Dudchenko GP1
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Affiliations:
- Issue: Vol 6, No 2 (2009)
- Pages: 20-22
- Section: Articles
- URL: https://journals.eco-vector.com/1994-9480/article/view/118031
- ID: 118031
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Abstract
Here we have examined by bisulfite sequencing the methylation status of the following tumor-suppressor genes: RASSF1A, BRCA1 and HIN1. For this study we used genomic DNA from five different breast cancer cell lines (MDA231, MDA435, MCF7, T47D, HS-578T) and five different ovarian cancer cell lines (A2780, SKOV3, OVCAR10, OVCAR5, OVCAR3)
as well as from normal tissue.
We found promoter region of RASSF1A and HIN1 to be hypermethylated in several cancer cell lines in both types of cancer. At the same time the promoter region of BRCA1 was unmethylated in all analyzed cancer cell lines.
Keywords
About the authors
A A Potapova
Oleg Vladimirovich Ostrovskiy
Email: ol.ostr@gmail.com
G P Dudchenko
A A Potapova
O V Ostrovskiy
G P Dudchenko
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