Vol 6, No 1 (2018)



Mayorova A.V., Syisuev B.B., Hanalieva I.A., Vihrova I.V.


The effectiveness of reparation is now characterized not only in terms of wound healing. The aesthetic result is also of great significance. Besides, it is important to ensure the comfort of medication, in order to combine the optimal therapy and the quality of life of the patient. The aim of the study was the literature review on the effective wound treatment with the help of modern dressings, including their assortment and prospects for improvement. Materials and methods. The materials of the study were reliable literary sources containing information about wounds, pathogenesis, the stages of the wound process and its possible violations; the assortment of modern wound coverings and the composition of biologically active substances that have a pharmacological effect; the results of preclinical and clinical trials and their prospects of use in aesthetic medicine. The research was carried out using the information retrieval and library databases (eLIBRARY, Cyberleninka), technical information. The research methods used in the work are: informational, analytical, descriptive. Results and discussion. As a result of generalization and analysis of modern publications devoted to the effective wound treatment, modern views on the wound process, wound coverings, their assortment and compositions are described. The results of pharmacological tests as well as the properties of biologically active substances and carrier polymers used are also presented. It is noted that the prospective compounds for including with the wound coverings and external drugs affecting the repair process at different stages of the wound process are tizol, bischofite, peptides (1-β-interleukin), ectoin. Conclusion. Thus, it has been established that modern wound dressings and dressings containing the substances of different pharmacological groups are offered for effective therapy: antiseptics, anesthetics, repair stimulators, antioxidants that affect different stages and elements of the wound process. Their use can be required in cosmetology. Improving the composition of wound coverings is relevant. The wound coverings based on biopolymers containing interleukins-1, ectoin, hyaluronic acid, bischofite and tizol are of great interest. 
Pharmacy & Pharmacology. 2018;6(1):4-32
pages 4-32 views


Bubenchikova V.N., Stepnova I.V.


The plants of the Hawkweed genus (Picris L.) belong to the Astrologic (Complex) family – Asteraceae (Compositae) and are used in folk medicine as diuretic, choleretic, laxative, analgesic, sudorific, emollient remedies. However, their chemical composition has not been studied sufficiently. Among other factors, in the process of the study sesquiterpene lactones were found in the aerial, including inflorescences, and subterranean parts; β-sitosterol was found in the leaves, and triterpenoids were found in the subterranean part of the plant. The aim of this work was the study of the qualitative composition and quantitative contents of biologically active substances of the Hawkweed herb. Materials and methods. As a material for the study, the aerial part of Hawkweed (Picris L.) was chosen. It was harvested in the vicinity of the city of Kursk in August 2016 and dried by air-shadow method. For the qualitative and quantitative analysis biologically active substances were extracted by various extractants (water, hydroalcoholic solutions, chloroform, hexane), and studied using conventional methods of phytochemical analysis. Results and discussion. The presence of carbohydrates (free and bound sugars, polysaccharides), organic acids, including ascorbic acid, phenolic compounds (tannins, flavonoids, coumarins, hydroxycinnamic acids), triterpene saponins, carotenoids has been established. The quantitative contents of water-soluble polysaccharides, pectin substances, organic acids, ascorbic acid, tannins, flavonoids, hydroxycinnamic acids, triterpene saponins, carotenoids have been determined. It was established that hydroxycinnamic acids, flavonoids, water-soluble polysaccharides, pectin substances represent the greatest significance of the investigated compounds of the Hawkweed herb. Conclusion. The carried out researches show that further phytochemical study of the Hawkweed herb is promising in order to study the possibility of its use in scientific medicine and the production of modern phytopreparations on its basis. 
Pharmacy & Pharmacology. 2018;6(1):33-46
pages 33-46 views


Salimova E.L., Konon A.D., Truhin V.P., Krasilnikov I.V.


Despite the world practice, in the Russian Federation immunization against hemophilic infection is carried out only for the children from risk groups, which may be due to the lack of production of this vaccine in the Russian Federation. Therefore, the development of technology for the preparation of polyribosylribitol phosphate (PRP, the active substance of the Hib vaccine) remains relevant. Earlier, the employees of the federal state unitary enterprise “Saint-Petersburg scientific research institute of vaccines and serums and the enterprise for the production of bacterial preparations” of Federal medical and biologic agency of Russia isolated and identified the strain of Haemophilus influenzae SPB type b B-7884. The conditions for cultivation of the strain B-7884 and primary isolation of PRP from the culture liquid were arranged. The aim of the work was to study the possibility of excluding the centrifugation stage for separating H. influenzae SPB type b B-7884 biomass before clarification on the cascade of filter discs, and the development of subsequent stages of PRP isolation from the obtained intermediate product. Materials and methods. PRP isolation from an inactivated culture liquid of strain B-7884 was carried out by centrifugation, filtration, precipitation, homogenization, extraction under various conditions. The concentration of PRP in the intermediate products was determined by the orcinol method, the identity was determined by the latex agglutination test. Results and discussion. The optimal scheme for isolation and purification of the active pharmaceutical ingredient PRP, including filtration through the cascade of deep filters Zeta plus LP 60 (0.3–0.6 μm) and Zeta plus LP 90 (0.1–0.3 μm), concentration and diafiltration on ultrafiltration cassettes with nominal molecular weight cut-off of 30 kDa, precipitation with 10% cetyltrimethylammonium bromide solution (5% v/v), with subsequent homogenization of the obtained precipitate (in the presence of 12.5% of ethanol from the concentrate volume) and extraction (32.5% ethanol), filtration through depth filters Zeta Plus SP 30 and carbon filters Zeta Plus Carbon R53, and precipitation by 1.0% (v/v) 4.0 M sodium chloride solution and freezing at –(20 ± 2)°C, was developed. Conclusion. The obtained results will become the basis for the further development of the polysaccharide substance and allow us to proceed to the next stage in the development of the vaccine against Haemophilus influenzae type b – conjugation with the carrier protein. 
Pharmacy & Pharmacology. 2018;6(1):47-62
pages 47-62 views


Karazhan N.V.


Helichrysum arenarium flowers are medical plant raw materials with a high concentration of flavonoids (2.5– 5.0%). As compounds of this group of biologically active substances often possess antiallergic activity, Helichrysum arenarium flowers are a potential antiallergic medicine. The aim is to study an antiallergic activity of Helichrysum arenarium flowers infusion on the model of mast cells degranulation in vitro. Materials and methods. Helichrysum arenarium flowers infusion was made in accordance with the requirements of the State Pharmacopoeia of the Republic of Belarus. Mast cells were obtained from the abdominal cavity of inbred mice weighing 20–25 g. Experimental animals were divided into three groups of 5 individuals in each. The intact group was not subjected to any manipulation for 14 days. The test group was sensitized with the epidermal allergen from the cat’s fur. The placebo group was subjected to the same manipulations as the test group, by phosphate buffered saline pH 7.4 for sensitization instead of the allergen. Results and discussion. The addition of Helichrysum arenarium flowers infusion to the mast cells of the intact group and the placebo group did not affect the percentage of the mast cells degranulation (p>0.05), indicating that the animals of the intact group and the placebo group did not have a hypersensitivity to the components of the medicine under investigation. In the presence of Helichrysum arenarium flowers the percentage of the mast cells degranulation of the test group decreased in comparison with the percentage of the mast cells degranulation in the presence of the allergen from 23.6±2.1% to 8.1–16.2% (p<0.05). Semi-effective concentration of Helichrysum arenarium flowers infusion was 1.33±0.04 mg / ml. The stabilizing effect on the mast cells degranulation of the Helichrysum arenarium flowers infusion was statistically significantly lower than the stabilizing effect of the Bidens tripartita herb infusion. Conclusion. Helichrysum arenarium flowers infusion has a pronounced dose-dependent stabilizing effect on the mast cells of male mice in vitro. Nevertheless, the stabilizing effect of Helichrysum arenarium flowers infusion on the mast cells was less pronounced than the stabilizing effect of Bidens tripartita herb infusion. 
Pharmacy & Pharmacology. 2018;6(1):63-72
pages 63-72 views


Luzhnova S.A., Voronkov A.V., Kodonidi I.P., Gabitova N.M., Billel S.


In various countries of the world, for at least last fifteen years, in the structure of nosocomial infections the leading position has been occupied by gram-negative pathogens, Escherichia coli in particular. The data of the World Health Organization testify the increasing resistance of this pathogen to antibiotics, which determines the relevance of creating and developing new antimicrobial agents. Primary screening of new 1.3-diazinon-4 derivatives and their non-cyclic precursors made it possible to identify the leader compounds capable of active suppressing the growth of E. coli and promising for the further study. The aim of the study is to investigate the activity of the compounds under laboratory ciphers of PYaTs4, PYaTs6, PYaTs7, PJTd8, PYaTd9, PYaTd15 with respect to Escherichia coli. Materials and methods. The study of the activity of the compounds was carried out on the basis of collection and freshly isolated strains from patients with different pathologies. Before the inoculation all the strains were identified by means of the BIOMIC V3 software complex (Giles Scientific, USA) to confirm their belonging to the genus and species. The strains used in the work were previously tested for susceptibility to antibacterial drugs by the disk method to assess the presence or absence of resistance. The activity of the new compounds was studied by the serial dilution method. Results and discussion. The investigation of the sensitivity of the strains by the disk method to antibacterial drugs used in the etiotropic therapy of infections caused by Escherichia coli revealed microorganisms with multiple drug resistance. The analysis of the inoculation of the strains showed that all the studied compounds have the ability to suppress the growth of E. coli being both collectively and freshly isolated from patients with various pathologies. Conclusion. The substances under the laboratory ciphers of PYaTd8 and PYaTs4 are the most active in respect to the studied strains. At the same time, the MIC50 (minimum inhibitory concentration) of all the compounds does not exceed 10–20 μg / ml, which makes them promising for the further study. 
Pharmacy & Pharmacology. 2018;6(1):73-85
pages 73-85 views


Bulgakov A.L., Kosmakov R.V.


This article examines the causes for creating alliances between manufacturers and drug developers. The attention has also been paid to the classification of such partnerships depending on the level of integration. The classification is necessary for further study of relations in the alliances. It is important to understand how economically effective and justified such partnerships are. The aim of the study is to determine the reasons for creating partnerships between drug developers and manufacturers, and to work out a classification of partnerships in the field of pharmacology. Materials and methods. The study analyzes existing alliances, partnerships of Big Pharma companies such as Bayer AG, Merck & Co, Pfizer. Results and discussion. The variety of forms of partnerships allows all participants in this market to get from cooperation the benefits they need. Some seek to reduce risk, others look forward to gaining access to new research opportunities, the third seek to share knowledge for greater productivity. However, there is always the opposite side, they have to sacrifice something to get a market advantage. For example, increased integration leads to a reduction in the risk of drug creation, but also leads to a decrease in the company’s independence. Conclusion. The results of this study can be used later in the study of the financial effectiveness of pharmaceutical partnerships, as well as in exploring the causes of alliances’ collapses. Any partnership has its value and can be considered as an intangible asset of the company. However, at the moment, there is no universal way of assessing this type of active assets, therefore, classification is a key step in creating a model for determining the value of a partnership. 
Pharmacy & Pharmacology. 2018;6(1):86-98
pages 86-98 views

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