卷 4, 编号 6 (2016)
- 年: 2016
- 文章: 5
- URL: https://journals.eco-vector.com/2307-9266/issue/view/5622
Articles
THE CURRENT STATE OF STUDY OF PLANTS OF HEDYSARUM L. FLORAE OF THE CAUCASUS
摘要
The article presents an overview of the ecological-botanical research and the current state of knowledge of species of the genus Hedysarum L. of the legume family (Fabaceae) grown in the Caucasus.The aim of the study was the review of the information contained in open sources about the basic ecological and Botanical characteristics, geographic specie of habitat and phytocoenotic specie of species of the genus Hedysarum growing in the Caucasus.Materials and methods. The study was conducted using information retrieval (PubMed, ScholarGoogle), library databases (eLibrary, Cyberleninca, RGB, VINITI, SOCIONET, ReseachGate, PubMed, Web of Sciences, SCOPUS, RNMJ.RU, UlrichsWEB, EBSCO, Scientific library of the Botanical Institute of RAS) as well as the results of our research.Results: the review study of species of the genus Hedysarum L. of the legume family (Fabaceae) grown in the Caucasus.Conclusion: the results of the analysis of regional floras, morphological characteristics, the main ecological and botanical characteristics of these 16 species, the geographic specie of habitat, phytocenotic specie are presented.
Pharmacy & Pharmacology. 2016;4(6):4-32
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AMINOACID COMPOSITION OF SOME SPECIES FROM INULA GENUS
摘要
The majority of medicinal plants and medicinal plant raw materials are understudied pharmacgostically. These plants include species from Inula genus, which range in Russia amounts to up to 40 species. Rhizomes and roots of the Inula helenium L. are broadly applied in scientific and traditional medicine. They have expectorate, styptic, and anti-inflammatory properties.The purpose of the study was to determine the amino-acid composition of some species from Inula genus (Inula germanica, Inula ensifolia, Inula aspera, Inula orientalis), which grow in different regions if the North Caucasus.Methods. The studies were carried out using AAA 400 amino acid analyzer, highly specialized automatized liquid chromatographer with computer management. Aboveground parts of the plants, gathered in mass blossom phase from wild-growing plants and then dried out were the objects of the study.Results. For the first time the amino acid composition and raw protein of some species from Inula genus was determined (Inula germanica, Inula ensifolia, Inula aspera, Inula orientalis), 16 amino acids were discovered, 7 of which were essential, and raw proteins which substantival composition amounted to 16.19% in Inula germanica, 10.78% in Inula ensifolia, Inula aspera (11.15%), Inula orientalis (13.94%).Conclusion. The results of the studies conducted broaden the data about amino acids and protein composition and quantitative content in the species from Inula genus and can be used to develop methods of analysis of the drugs, obtained from these plants.
Pharmacy & Pharmacology. 2016;4(6):33-43
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THE TECHNOLOGY OF OBTAINING OF HEMOSTATIC GEL OF LAGODEN ON THE BASE OF CARBOPOL
摘要
We are conducting a series of experiments for the development of technology of Lagoden hemostatic gel obtaining. We studied different bases for the gel obtaining. According to the results, carbopol was selected as a gelling agent. By studying the change in the rheological properties of the gel and technological basis, a series of experiments were done to determine the concentration of a gelling agent and a neutralizing agent. We worked on an optimal composition of the drug and a method for producing it in the technological process. Initially some substances like carbopol, sodium hydroxide, glycerin, purified water, and preservatives were used. Lagoden is a sodium salt trihydrate 3,16,18-trihydroxy-9,13-epoxilabdan-15-carboxylic acid. Lagoden is obtained by processing of Inebrin substance, which is obtained from a Lagochilus inebriansplant. Lagochilus inebrians has been used in folk medicine for a long time.The aim of the work was to develop technology of obtaining of the soft drug form of Lagoden.Methods. Chemical reactions, me-thods of soft drug forms obtaining were used in the study.Results. Influence of different gel components on Lagoden gel were tested through various experiments. After studying of gel characteristics which on the basis of carbopol-934 its alternative concentration for the formation of optimal gelwas defined. The experiments determined that the carbopol gel retained its physical state in the range of 5.5
Pharmacy & Pharmacology. 2016;4(6):44-53
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Method development and validation for determination of antihemolytic activity of eculizumab (Soliris)
摘要
Bioanalytical methods are applied at the development and test of drugs as well as at the step of pharmaceutic products issue. Drugs and excipients quality estimation is made by means of precise and reproducible methods. Accuracy and reproducibility of a method is established during validation which is obligatory for medicine registration. The principal task of method validation is experimental evidence of its suitability for the objectives to be achieved. Validation of bioanalytical methods is one of the elements of the whole medicine production validation [1, 2].The aim of research: to validate a method for determination of specific anti-hemolytic activity of eculizumab developed in LCC “IBC Generium”.Materials and methods: eculizumab, antibody-sensitized chicken erythrocytes, complement-containing human serum.Results. We demonstrated the specificity of the method and its correspondence to criteria of accuracy (103.0±1.4)%, robustness (CV – 11.5%), repeatability (CV – (4.9±0.9)%), reproducibility (CV – (3.5±0.4)%), and linearity (k -1.0275; R2 – 0.9975) during validation. The system validity (equipment, materials, analytical operations and analyzed samples) was confirmed for true results obtaining during validation.Results discussion. Experimental evidence of suitability of the method for eculizumab specific activity assessment was obtained in course of validation. The simplicity of the method allows obtaining accurate results in other laboratories. The developed method can be used not only for specific activity of Soliris determination but also for other pharmaceutical substances and drugs based on antibodies specific to human complement C5.
Pharmacy & Pharmacology. 2016;4(6):54-71
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ADMINISTRATIVE AND ORGANIZATIONAL ASPECTS OF FUNCTIONING OF THE SYSTEM OF AVAILABILITY NARCOTIC AND PSYCHOTROPIC MEDICINAL PREPARATIONS FOR PATIENTS WHO NEED THEM
摘要
Narcotic drugs and psychotropic substances still remain indispensable in medicine. One of the top priorities of health care is to ensure the required availability of this group of drugs. The development of an effective system which can ensure crucially important availability of narcotic and psychotropic agents to people who need them for medical purposes must rely on the identification and analysis of the nature and specificity of the problems related to them as well as the ways of solving these problems. ThePurpose of the Study was to explore the constituents of the system ensuring medicinal availability of narcotic and psychotropic agents and to identify the factors hindering the availability of this group of medicinal agents.Materials and Methods. The study involved the systematic review analysis of the scientific publications and guidance documents regulating various aspects of activities involving the turnover of controlled drugs, and the results of surveys and interviews in which medical and pharmaceutical professionals were engaged. The study employed the methods of content analysis, logical and functional analysis, etc.Results and Discussion. The system of narcotic and psychotropic drug (NPD) availability for patients who need them is currently going through reforms which have been conditioned by low accessibilty of these drugs when used for medical purposes. Those engaged in the drug supply system including medical and pharmaceutical professionals point out that excessive bureaucratization of the system of NPD supply and availability, complexity of documentation and paperwork reporting thedrug flow, costliness of these activities, and strict liability account for low availability of such drugs. To ensure the rights of the citizens to relieve pain due to diseases or medical interventions, a number of changes in the laws and regulations have been made. They have simplified the requirements for NPD turnover in regard to administering, prescribing and dispensing the drugs. However, the findings of the studies which have been conducted demonstrate that the massive reforms of the regulatory framework aiming to change the situation are not actually implemented. The specialists involved in the field prefer not to enforce new laws and make every possible effort to avoid using new regulations instead and search for the opportunities either to minimize the activities involving controlled medicinal agentsor to refuse them altogether. In this situation, patients who need opioid analgesics, even within a favourable regulatory framework, are facing a lot of problems when the drugs are prescribed or dispersed.Сonclusions: In this case, a solution of the problem of low availability of NPD must involve the improvement of administrative management of the availability system at the level of medical and pharmaceutical organizations.
Pharmacy & Pharmacology. 2016;4(6):72-83
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