OREXIN-B-CONTAINING NEURONS IN RAT HYPOTHALAMUS AFTER LIPOPOLYSACCHARIDE AND BOVINE SERUM ALBUMIN ADMINISTRATION
- Authors: Pugach VA1, Novikova NS1, Perekrest SV1, Korneva EA1
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Affiliations:
- Institute of Experimental Medicine
- Issue: Vol 16, No 1 (2016)
- Pages: 50-56
- Section: Articles
- URL: https://journals.eco-vector.com/MAJ/article/view/9480
- DOI: https://doi.org/10.17816/MAJ16150-56
- ID: 9480
Cite item
Abstract
Hypothalamic orexin-B-containing neurons reaction was investigated during several hours after administration of thymus-dependent (bovine serum albumin - BSA) and thymus-independent (lipopolysaccharide - LPS) antigen. Using immunohistochemical method we revealed changes in quantity of visualized orexin-B positive neurons and their relative optical density (ROD) only 1 hour after LPS injection. Four patterns of changes were distinguished: increase of neurons quantity; increase of neurons ROD; increase of both cells quantity and ROD; simultaneously neurons quantity increase and ROD decrease. Thus injection of T-independent antigen (LPS) causes multidirectional reaction of orexin-B-positive neurons, that could be explained with morphofunctional heterogeneity of these neurons. Administration of T-dependent antigen (BSA) does not change immunoreactivity of orexin-B-positive neurons during several hours after antigen application.
Keywords
Full Text
About the authors
V A Pugach
Institute of Experimental Medicine
N S Novikova
Institute of Experimental Medicine
Email: novikiem@gmail.com
S V Perekrest
Institute of Experimental Medicine
Email: perekrest.sv@gmail.com
E A Korneva
Institute of Experimental Medicine
Email: glandula_pinealis@mail.ru
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