OREXIN-B-CONTAINING NEURONS IN RAT HYPOTHALAMUS AFTER LIPOPOLYSACCHARIDE AND BOVINE SERUM ALBUMIN ADMINISTRATION
- Authors: Pugach VA1, Novikova NS1, Perekrest SV1, Korneva EA1
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Affiliations:
- Institute of Experimental Medicine
- Issue: Vol 16, No 1 (2016)
- Pages: 50-56
- Section: Articles
- Published: 15.03.2016
- URL: https://journals.eco-vector.com/MAJ/article/view/9480
- DOI: https://doi.org/10.17816/MAJ16150-56
- ID: 9480
Cite item
Abstract
Hypothalamic orexin-B-containing neurons reaction was investigated during several hours after administration of thymus-dependent (bovine serum albumin - BSA) and thymus-independent (lipopolysaccharide - LPS) antigen. Using immunohistochemical method we revealed changes in quantity of visualized orexin-B positive neurons and their relative optical density (ROD) only 1 hour after LPS injection. Four patterns of changes were distinguished: increase of neurons quantity; increase of neurons ROD; increase of both cells quantity and ROD; simultaneously neurons quantity increase and ROD decrease. Thus injection of T-independent antigen (LPS) causes multidirectional reaction of orexin-B-positive neurons, that could be explained with morphofunctional heterogeneity of these neurons. Administration of T-dependent antigen (BSA) does not change immunoreactivity of orexin-B-positive neurons during several hours after antigen application.
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About the authors
V A Pugach
Institute of Experimental Medicine
N S Novikova
Institute of Experimental Medicine
Email: novikiem@gmail.com
S V Perekrest
Institute of Experimental Medicine
Email: perekrest.sv@gmail.com
E A Korneva
Institute of Experimental Medicine
Email: glandula_pinealis@mail.ru
References
- Ma X. C., Oliver J., Horvath E., Phelps C. P. Cytokine and adrenal axis responses to endotoxin // Brain Res.- 2000.- Vol. 861.- P. 135-142.
- Martich G. D., Danner R. L., Coska M., Suffredini A. F. Detection of interleukin-8 and tumor necrosis factor in normal humans after intravenous endotoxin: The effects of anti-inflammatory agents // J. Exp. Med.- 1991.- Vol. 173.- P. 1021-1024.
- Shelag P. B. Interleukin-6-type cytokines in vivo: Regulated bioavailability // Proc. Soc. Exp. Biol Med.- 1996.- Vol. 213.- P. 238-247.
- Перекрест С. В., Гаврилов Ю. В., Абрамова Т. В., Новикова Н. С., Корнева Е. А. Активация клеток гипоталамических структур при введении антигенов различной природы (по экспрессии c-fos гена) // Медицинская иммунология.- 2006.- Т. 8, № 5-6.- С. 631-636.
- Wanner S. P., Yoshida K., Kulchitsky V. A., Ivanov A. I., Kanosue K., Romanovsky A. A. Lipopolysaccharide-Induced Neuronal Activation in the Paraventricular and Dorsomedial Hypothalamus Depends on Ambient Temperature // LoS ONE.- 2013.- № 8 (9).- P. 69-80.
- De Lecea L., Kilduff T. S., Peyron C., Gao X., Foye P. E., Danielson P. E., Fukuhara C., Battenberg E. L., Gautvik V. T., Bartlett F. S., Frankel W. N., van den Pol A. N., Bloom F. E., Gautvik K. M., Sutcliffe J. G. The hypocretins: hypothalamus-specific peptides with neuroex-citatory activity // Proc. Natl. Acad. Sci. USA.- 1998.- Vol. 95.- P. 322-327.
- Sakurai T., Amemiya A., Ishii M., Matsuzaki I., Chemelli R. M., Tanaka H., Williams S. C., Richarson J. A, Kozlowski G. P., Wilson S., Arch J. R., Buckingham R. E., Haynes A. C., Carr S. A., Annan R. S., McNulty D. E., Liu W. S., Terrett J. A., Elshourbagy N. A., Bergsma D. J., Yanagisawa M. Orexins and orexin receptors: a family of hypothalamic neuroprptides and G protein-coupled receptors that regulate feeding behavior // Cell.- 1998.- Vol. 92.- P. 573-585.
- Kukkonen J. P. Physiology of the orexinergic/hypocretinergic system: a revisit in 2012// Am. J.Physiol Cell Physiol.- 2013.- Vol. 304.- P. 2-32.
- Li J., Hu Z., de Lecea L. The hypocretins/orexins: integrators of multiple physiological functions // Brit. J.Pharmacol.- 2014.- Vol. 171.- P. 332-350.
- Tsunematsu T., Yamanaka A. The Role of Orexin/Hypocretin in the Central Nervous System and Peripheral Tissues // Vitamins and Hormones.- 2012.- Vol. 89.- P. 20-29.
- Phelps C., Chen L.-T. Brain response to endotoxin // Cytokines and the brain / еd. by C. Phelps and E. Korneva.- 2008.- P. 435-455.
- Perekrest S. V., Abramova T. V., Novikova N. S., Loskutov Yu. V., Rogers V. J., Korneva E. A. Changes in immunoreactivity of orexin-A-positive neurons after an intravenous lipopolysaccharide injection // Medical Science Monitoring.- 2008.- Vol. 14, № 7.- P. 127-133.
- Перекрест С. В., Абрамова Т. В., Новикова Н. С. Сравнительный анализ реакции орексинсодержащих нейронов гипоталамуса крысы при введении различных доз липополисахарида // Российский физиологический журнал им. И. М. Сеченова.- 2009.- Т. 95, № 12.- С. 1336-1345.
- Palomba M., SekeEtet P. F., Varonesi C. Effect of inflammatory challenge on hypothalamic neurons expressing orexinergic and melanin-concentrating hormone // Neuroscience Letters.- 2014.- Vol. 570.- P. 47-52.
- Taheri S., Sunter D., Dakin C., Moyes S., Seal L., Gardiner J., Rossi M., Ghatei M., Bloom S. R. Diurnal variation in orexin A immunoreactivity and prepro-orexin mRNA in the rat central nervous system // Neuroscience Letters.- 2000.- Vol. 279, № 1/2.- P. 109-112.
- Swanson L. W. Brain maps: structure of the Rat Brain.- 2nd revised ed. -Amsterdam: Elsevier,1998.- P. 267.
- Contreras C., Gonzalez F., Ferno J, Dieguez C., Rahmouni K., Nogueiras R., Lopez M. The brain and brown fat // Ann. of Medicine.- 2015.- P. 150-168.
- Morrison S. F., Madden C. J., Tupone D. An orexinergic projection from perifornical hypothalamus to raphe pallidus increases rat brown adipose tissue thermogenesis // Adipocytе.- 2012.- Vol. 1-2.- P. 116-120.
- Yasuda T., Masaki T., Kakuma T., Hara M., Nawata T., Katsuragi I., Yoshimatsu H. Dual Regulatory Effects of Orexins on Sympathetic Nerve Activity Innervating Brown Adipose Tissue in Rats // Endocrinology.- 2001.- Vol. 146, № 6.- P. 2744-2748.
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