Asymmetry in the content of brain monoamines of BALB/c mice reared in social isolation conditions



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Abstract

The effects of long-term social isolation on the content and metabolism of dopamine and serotonin systems were studied in symmetrical brain structures of BALB/c male mice. With HPLC the contents of dopamine (DA), serotonin (5-HT) and their metabolites dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindolacetic acid (5-HIAA) were measured in the cortex, hippocampus and striatum of both the right and the left hemispheres of the brain in mice reared in groups and social isolation. The isolated mice were characterized by reduced level of DA in the left striatum and elevated level of 5-HIAA and ratio 5-HIAA/5-HT in the right striatum. In the hippocampus of isolated mice, the activation of both DA-ergic and 5-HT-ergic systems was observed, that is the high level of DA and DOPAC in the left hippocampus and the elevated level of 5-HT in both hemispheres and of 5-HIAA in the right hippocampus were registered. On the other hand, the reduction of both DA-ergic and 5-HT-ergic systems activity was shown to be in the right hemisphere. The decreased concentration of DOPAC and ratio DOPAC/DA in the right cortex were observed as well. As to 5-HT-ergic system, the reduced level of 5-HT in the both cortex of the hemispheres as well as 5-HIAA in the right hemisphere of isolated mice was determined. The phenomenon of interhemispheric asymmetry was revealed in the hippocampus only, which was characterized by the increased DA-ergic activity in the left hippocampus but not in the striatum and the cortex.

About the authors

Inessa Vladimirovna Karpova

I. P. Pavlov State Medical University

Email: inessa.karpova@gmail.com
PhD (Physiology), Assistant Professor, Dept. of Normal Physiology

Vladimir Vladimirovich Mikheyev

S. M. Kirov Military Medical Academy

Email: vmikheev58@gmail.com
Doctor of Biol. Sci. (Pharmacology), Assistant Professor, Dept. of Pharmacology

Yevgeniy Rudolfovich Bychkov

Institute of Experimental Medicine NWB RAMS

PhD (Pathophysiology), Senior Researcher, S. V. Anichkov Dept. of NeuroPharmacology

Andrey Andreyevich Lebedev

Institute of Experimental Medicine NWB RAMS

Email: aalebedev-iem@yandex.ru
Doctor of Biol. Sci. (Pharmacology), Professor, Senior Researcher, S. V. Anichkov Dept. of NeuroPharmacology

Petr Dmitriyevich Shabanov

Institute of Experimental Medicine NWB RAMS

Email: pdshabanov@mail.ru
Dr. Med. Sci. (Pharmacology), Professor, Head, Dept. of Neuropharmacology

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Copyright (c) 2012 Karpova I.V., Mikheyev V.V., Bychkov Y.R., Lebedev A.A., Shabanov P.D.

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