The efficiency of molecular markers of the SKr suppressor gene that determines the crossability of common wheat with rye

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Abstract

BACKGROUND: Among the genes involved in the control of crossing between common wheat and rye, the dominant suppressor SKr (Suppressor of crossability) having the major effect on inhibiting crossability is the most studied. DNA-markers have been developed for this gene. There are cases of successful application of some of them in wheat breeding, but there is practically no information about their diagnostic efficiency in screening of ex situ collections.

MATERIALS AND METHODS: To evaluate the effectiveness of SKr markers Xcfb341, TGlc2, gene12 и gene13 to identify forms with high crossability with rye, we studied 103 bread wheat accessions from the VIR collection with different seed set (0–93%) after pollinated with rye.

RESULTS: Efficiency in detection crossable forms (upper 15%) was demonstrated by markers Xcfb341, TGlc2 and gene12. No significant allele-trait association was found for gene13 marker. Ten haplotypes were identified based on four markers. Five haplotypes were revealed for accessions from China, while two of them were associated with “high crossability”. Eleven accessions including the line L6-HSR were heterogenic in markers. For this line the relationship of diagnostic fragments with the crossability level observed in the field has been experimentally confirmed.

CONCLUSIONS: The SKr gene markers studied can be used for accessions searching at ex situ collections that potentially have high crossability with rye, for controlling the retention of recessive skr alleles when regenerating seed accessions, as well as in breeding programs.

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About the authors

Igor V. Porotnikov

N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Author for correspondence.
Email: i.v.porotnikov@gmail.com
ORCID iD: 0000-0001-5841-8803
SPIN-code: 9010-0859
Scopus Author ID: 57219994008

Junior Research Associate

Russian Federation, Saint Petersburg

Valentina P. Puykkenen

N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: tina7@yandex.ru
Scopus Author ID: 57211942307

Senior Research Associate

Russian Federation, Saint Petersburg

Olga Yu. Antonova

N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: olgaant326@mail.ru
ORCID iD: 0000-0001-8334-8069
SPIN-code: 9255-6449
Scopus Author ID: 23391684100

Cand. Sci. (Med.), Head of laboratory of Molecular breeding and DNA-genotyping

Russian Federation, Saint Petersburg

Olga P. Mitrofanova

N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: o.mitrofanova@vir.nw.ru
ORCID iD: 0000-0002-9171-2964
Scopus Author ID: 6602647964

Dr. Sci. (Med.), Main Research Associate

Russian Federation, Saint Petersburg

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Supplementary files

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2. Fig. 1. Incidence (%) of Xcfb341, TGlc2, gene12, and gene13 alleles in the soft wheat genotypes studied. For TGlc2 and gene13, the alleles detected in all genotypes are not given

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3. Fig. 2. Diversity of amplified DNA fragments detected using Xcfb341 (a) and gene12 (b) markers in the soft wheat genotypes studied. 1, 2 - Chinese Spring; 3, 4 - Khludovskaya; 5 - Punjab Tipe 8A; 6 - Akadaruma; 7, 8 - k-34883; 9 - Siberian Yartsevskaya (p-1); 10 - negative control (H2O); 11 - Red Star; 12 - Bage; 13, 14 - k-42052 (p-1, 2); 15 - Punjab Tipe 8A; 16 - L16-HSR. M - molecular weight marker 100 bp + 1.5 Kb (SibEnzyme)

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4. Fig. 3. Diversity of amplified DNA fragments detected using TGlc2 (a) and gene13 (b) markers in the soft wheat genotypes studied. 1 - negative control (H2O); 2 - Courtot; 3 - L42-HSR; 4 - Chinese Spring; 5 - Moro of Sind (p-2); 6 - Akadaruma; 7 - Nemchinovskaya 24; 8 - Punjab Tipe 8A; 9 - Siberian Yartsevskaya (p-1)

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5. Fig. 4. Distribution of 114 studied genotypes with different haplotypes depending on crossing with rye

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