Creation of new pichia pastoris strains for recombinant protein production

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Abstract


Pichia pastoris yeasts are widely used as a production platform for heterologous proteins. Wide biotechnological use of these yeasts is determined by simplicity of cultivation, cheap media and ability to provide posttranslational modifications. Basic approaches for enhancement of the recombinant protein outcome constitue increasing number of gene copies, which encode target protein, as well as co-expression of supporting factors for protein folding, processing and secretion. Development of relevant plasmids and auxotrophic strains is essential for solving these tasks. In this study, we report plasmids and strains collection, whichwill allow to conduct integration of multiple foreign genes in P. pastoris genome

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About the authors

Dmitriy Mikhaylovich Muzaev

Saint Petersburg State University

Email: dmmuzaev@yandex.ru
master, Dept. Genetics and Biotechnology

Andrey Mikhaylovich Rumyantsev

Saint Petersburg State University

Email: rumyantsev-am@mail.ru
master, Dept. Genetics and Biotechnology

Elena Viktorovna Sambuk

Saint Petersburg State University

Email: esambuk@mail.ru
Dr. Sci. Biol, professor, Dept. Genetics and Biotechnology

Marina Vladimirovna Padkina

Saint Petersburg State University

Email: mpadkina@mail.ru
Dr. Sci. Biol, professor, Dept. Genetics and Biotechnology

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Copyright (c) 2015 Muzaev D.M., Rumyantsev A.M., Sambuk E.V., Padkina M.V.

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