The new taxonomic marker of nodulation bacteria of Rhizobium genus and its evolution



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Abstract

The new taxonomic marker (hin-region) has been proposed, which gives possibility for Rhizobium bacteria study on “species — group of strains” level. Using this marker the groups of Rhizobium strains were determined, which could not be distinguished with other methods, and these results correlated with evolutionary similarity of the bacteria. The developed approach for creating marker systems allows to carry out effective inventory of inter- and intraspecies genetic diversity of nodulating bacteria and to evaluate perspectives of their use in agriculture. The proposed marker system was used for description of Rhizobium bacteria samples isolated from various ecological-geographical regions of Ukraine.

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About the authors

Vasily S Zotov

A.N. Bach Institute of Biochemistry RAS, Moscow, RF

Email: adni83@yandex.ru

Natalia V Punina

Research Centre for Medical Genetics, Moscow, RF

Email: hin-enkelte@yandex.ru

Sofia A Khapchaeva

A.N. Bach Institute of Biochemistry RAS, Moscow, RF

Email: hapchaeva90@mail.ru

Svetlana V Didovich

Institute of Agriculture of Crimea of National Academy of Agriculture Sciences of Ukraine, Simferopol, Ukraine

Email: sv-alex.68@mail.ru

Tatyana N Melnichuk

Institute of Agriculture of Crimea of National Academy of Agriculture Sciences of Ukraine, Simferopol, Ukraine

Email: melnichuk tn@ukr.net

Aleksey F Topunov

A.N. Bach Institute of Biochemistry RAS, Moscow, RF

Email: aftopunov@yandex.ru

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Copyright (c) 2012 Zotov V.S., Punina N.V., Khapchaeva S.A., Didovich S.V., Melnichuk T.N., Topunov A.F.

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