A molecular genetic research of the Triticum sinskajae A. Filat. et Kurk. by RAPD analysis and by comparing the nucleotide sequences of the variable intergenic region of the petN-trnC-GCA chloroplast genome and intron of the histone H3.2 gene

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Abstract


Background. Triticum sinskajae A. Filat. et Kurk. was discovered in the early 70th in the last century at the regular reproduction in the Central Asian and Dagestan VIR-stations of T. monococcum samples.

Materials and methods. The objects of the study were 4 species of diploid wheat — Triticum urartu Thum. ex Gandil. (lines k-62477, k-62465), Triticum monococcum L. (lines k-20970, k-39471), Triticum boeoticum Boiss. (lines k-59161, k-28132, k-40118) and Triticum sinskajae A. Filat. et Kurk. (line k-48993).

Results. We found differences between T. sinskajaeand T. monococcum in the variable region of the histone gene H3.2, and the RAPD analysis showed the presence of unique polymorphic loci in T. sinskajae.

Conclusion. In gene ral, T. boeoticum, T. monococcum, and T. sinskajae are most likely to be closely related species of diploid wheat, whereas T. urartu is quite significantly different from them.


Azat R. Kuluev

Author for correspondence.
kuluev.azat91@yandex.ru
Ufa Federal Research Centre of the Russian Academy of Sciences, Institute of Biochemistry and Genetics
Russian Federation, Ufa

Post-graduate Student

Rustam T. Matnijazov

rmat@mail.ru
Ufa Federal Research Centre of the Russian Academy of Sciences, Institute of Biochemistry and Genetics
Russian Federation, Ufa

Ph.D, Researcher

Bulat R. Kuluev

kuluev@bk.ru
Ufa Federal Research Centre of the Russian Academy of Sciences, Institute of Biochemistry and Genetics
Russian Federation, Ufa

Doctor of Biology, Senior Researcher

Alexey V. Chemeris

chemeris@anrb.ru
Ufa Federal Research Centre of the Russian Academy of Sciences, Institute of Biochemistry and Genetics
Russian Federation, Ufa

Doctor of Biology, Professor, Chief Researcher

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Supplementary files

Supplementary Files Action
1. Fig. 1. A phylogenetic tree constructed from the results of a RAPD-analysis of diploid wheats View (21KB) Indexing metadata
2. Fig. 2. A phylogenetic tree constructed from the analysis of nucleotide sequences of the variable region of the gene encoding H3.2 histone diploid wheat View (85KB) Indexing metadata
3. Fig. 3. A phylogenetic tree constructed by analysis of the nucleotide sequences of the variable intergenic region of petN-trnC-GCA of the chloroplast genome of diploid wheats View (179KB) Indexing metadata

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Copyright (c) 2018 Kuluev A.R., Matnijazov R.T., Kuluev B.R., Chemeris A.V.

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