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Morphological and immunohistochemical parameters of chron-ic placental insufficiency in preeclampsia
- Authors: Kulida L.V.1, Rokotyanskaya E.A.1, Panova I.A.1, Malyishkina A.I.1, Protsenko E.V.1, Maisina A.I.1
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Affiliations:
- Gorodkov Ivanovo Research Institute of Maternity and Childhood
- Issue: Vol 28, No 4 (2020)
- Pages: 449-461
- Section: Original study
- URL: https://journals.eco-vector.com/pavlovj/article/view/34833
- DOI: https://doi.org/10.23888/PAVLOVJ2020284449-461
- ID: 34833
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Abstract
Aim. To determine the morphological parameters of chronic placental insufficiency in pregnancy complicated by preeclampsia and in women with chronic arterial hypertension (CAH).
Materials and Methods. Historical data and peculiarities of pregnancy and childbirth in women with hypertensive disorders were analyzed in this work. A review histology of 40 placentas in moderate preeclampsia, 40 placentas in severe preeclampsia, and 35 placentas of women with CAH and associated preeclampsia was performed. The control group consisted of 20 placentas of women with uncomplicated pregnancies and without hypertensive disorders. Immunohistochemical examinations were performed on paraffin sections according to standard methods using primary goat antibodies to annexin V (R-20, sc-1929) and rabbit antibodies to erythropoietin (H-162, sc-7556) in a working dilution of 1:200 with the Super Sensitive IHC polymer detection system.
Results. The results of the pathomorphological examination of the placentas of women with hypertensive disorders showed two forms of chronic placental insufficiency. The defining form of placental insufficiency in women with CAH and associated preeclampsia was fetoplacental insufficiency while that in preeclampsia of moderate and high severity was the uteroplacental form of chronic placental insufficiency. On the basis of the study of the dynamics of the expression of annexin V and erythropoietin, the morphological parameters of placental compensatory potential and placental hemostasis disorders in hypertensive disorders in pregnant women were determined.
Conclusion. The diagnostic morphological criteria for fetoplacental insufficiency in women with hypertensive disorders are a combination of maternal and fetal malperfusion with obliterative angiopathy of stem villi vessels. For the uteroplacental form, the criteria are the obliterative angiopathy of spiral arteries, placental hypoperfusion with the development of local hypoxia, and hemostatic disorders in the form of thrombosis of the intervillous space and villi infarcts.
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About the authors
Ludmila V. Kulida
Gorodkov Ivanovo Research Institute of Maternity and Childhood
Author for correspondence.
Email: kulida@mail.ru
ORCID iD: 0000-0001-8962-9048
SPIN-code: 6208-4487
ResearcherId: B-1080-2017
MD, PhD, Leading Researcher of the Pathomorphology and Electronic Microscopy Laboratory
Russian Federation, IvanovoElena A. Rokotyanskaya
Gorodkov Ivanovo Research Institute of Maternity and Childhood
Email: rokotyanskaya.ea@mail.ru
ORCID iD: 0000-0003-4660-7249
SPIN-code: 4464-5069
ResearcherId: B-3595-2017
MD, PhD, Assistant Professor of the Department of Obstetrics and Gynecology, Neonatology, Anesthesiology and Reanimatology
Russian Federation, IvanovoIrina A. Panova
Gorodkov Ivanovo Research Institute of Maternity and Childhood
Email: ia_panova@mail.ru
ORCID iD: 0000-0002-0828-6547
SPIN-code: 9507-9174
ResearcherId: A-9570-2017
MD, PhD, Assistant Professor, Head of the Obstetrics and Gynecology Department
Russian Federation, IvanovoAnna I. Malyishkina
Gorodkov Ivanovo Research Institute of Maternity and Childhood
Email: ivniimid@inbox.ru
ORCID iD: 0000-0002-1145-0563
SPIN-code: 7937-9125
ResearcherId: B-5680-2017
MD, PhD, Рrofessor, Director
Russian Federation, IvanovoElena V. Protsenko
Gorodkov Ivanovo Research Institute of Maternity and Childhood
Email: ivniimid@inbox.ru
ORCID iD: 0000-0003-0490-5686
SPIN-code: 1343-3881
ResearcherId: B-1147-2017
MD, PhD, Head of the Pathomorphology and Electronic Microscopy Laboratory
Russian Federation, IvanovoAlexandra I. Maisina
Gorodkov Ivanovo Research Institute of Maternity and Childhood
Email: mosay16@mail.ru
ORCID iD: 0000-0002-8738-4371
SPIN-code: 3026-3987
ResearcherId: AAR-1837-2020
PhD-Student of the Pathomorphology and Electronic Microscopy Laboratory
Russian Federation, IvanovoReferences
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