Morphological and immunohistochemical parameters of chron-ic placental insufficiency in preeclampsia

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Aim. To determine the morphological parameters of chronic placental insufficiency in pregnancy complicated by preeclampsia and in women with chronic arterial hypertension (CAH).

Materials and Methods. Historical data and peculiarities of pregnancy and childbirth in women with hypertensive disorders were analyzed in this work. A review histology of 40 placentas in moderate preeclampsia, 40 placentas in severe preeclampsia, and 35 placentas of women with CAH and associated preeclampsia was performed. The control group consisted of 20 placentas of women with uncomplicated pregnancies and without hypertensive disorders. Immunohistochemical examinations were performed on paraffin sections according to standard methods using primary goat antibodies to annexin V (R-20, sc-1929) and rabbit antibodies to erythropoietin (H-162, sc-7556) in a working dilution of 1:200 with the Super Sensitive IHC polymer detection system.

Results. The results of the pathomorphological examination of the placentas of women with hypertensive disorders showed two forms of chronic placental insufficiency. The defining form of placental insufficiency in women with CAH and associated preeclampsia was fetoplacental insufficiency while that in preeclampsia of moderate and high severity was the uteroplacental form of chronic placental insufficiency. On the basis of the study of the dynamics of the expression of annexin V and erythropoietin, the morphological parameters of placental compensatory potential and placental hemostasis disorders in hypertensive disorders in pregnant women were determined.

Conclusion. The diagnostic morphological criteria for fetoplacental insufficiency in women with hypertensive disorders are a combination of maternal and fetal malperfusion with obliterative angiopathy of stem villi vessels. For the uteroplacental form, the criteria are the obliterative angiopathy of spiral arteries, placental hypoperfusion with the development of local hypoxia, and hemostatic disorders in the form of thrombosis of the intervillous space and villi infarcts.

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作者简介

Ludmila Kulida

Gorodkov Ivanovo Research Institute of Maternity and Childhood

编辑信件的主要联系方式.
Email: kulida@mail.ru
ORCID iD: 0000-0001-8962-9048
SPIN 代码: 6208-4487
Researcher ID: B-1080-2017

MD, PhD, Leading Researcher of the Pathomorphology and Electronic Microscopy Laboratory

俄罗斯联邦, Ivanovo

Elena Rokotyanskaya

Gorodkov Ivanovo Research Institute of Maternity and Childhood

Email: rokotyanskaya.ea@mail.ru
ORCID iD: 0000-0003-4660-7249
SPIN 代码: 4464-5069
Researcher ID: B-3595-2017

MD, PhD, Assistant Professor of the Department of Obstetrics and Gynecology, Neonatology, Anesthesiology and Reanimatology

俄罗斯联邦, Ivanovo

Irina Panova

Gorodkov Ivanovo Research Institute of Maternity and Childhood

Email: ia_panova@mail.ru
ORCID iD: 0000-0002-0828-6547
SPIN 代码: 9507-9174
Researcher ID: A-9570-2017

MD, PhD, Assistant Professor, Head of the Obstetrics and Gynecology Department

俄罗斯联邦, Ivanovo

Anna Malyishkina

Gorodkov Ivanovo Research Institute of Maternity and Childhood

Email: ivniimid@inbox.ru
ORCID iD: 0000-0002-1145-0563
SPIN 代码: 7937-9125
Researcher ID: B-5680-2017

MD, PhD, Рrofessor, Director

俄罗斯联邦, Ivanovo

Elena Protsenko

Gorodkov Ivanovo Research Institute of Maternity and Childhood

Email: ivniimid@inbox.ru
ORCID iD: 0000-0003-0490-5686
SPIN 代码: 1343-3881
Researcher ID: B-1147-2017

MD, PhD, Head of the Pathomorphology and Electronic Microscopy Laboratory

俄罗斯联邦, Ivanovo

Alexandra Maisina

Gorodkov Ivanovo Research Institute of Maternity and Childhood

Email: mosay16@mail.ru
ORCID iD: 0000-0002-8738-4371
SPIN 代码: 3026-3987
Researcher ID: AAR-1837-2020

PhD-Student of the Pathomorphology and Electronic Microscopy Laboratory

俄罗斯联邦, Ivanovo

参考

  1. Voevodin SM, Shemanaeva TV, Shchegolev AI. Ultrasound and clinical-morphological evaluation of fetal-placental complex of pregnant women with intrauterine infection. Gynecology. 2015;(5):10-3. (In Russ).
  2. Strizhakov AN, Tezikov YuV, Lipatov IS, et al. Pathogenetic rationale for diagnosing and pregestational prevention of embryoplacental dysfunction. Voprosy Ginekologii, Akusherstva i Perinatologii. 2012;11(1):5-10. (In Russ).
  3. Lipatov IS, Tezikov YuV, Lineva YuV, et al. Pathogenetic mechanisms of placental insufficiency and preeclampsia. Akusherstvo i Ginekologiya. 2017; (9):64-71. (In Russ). doi: 10.18565/aig.2017.9.64-71
  4. Milovanov AP, Ozhiganova IN. Embryochorionic insufficiency: Anatomic and physiologic prerequisites, rationale, definitions and pathogenetic mechanisms. Arkhiv Pathologii. 2014;(3):4-8. (In Russ).
  5. Gazieva IA, Chistyakova GN, Remizova II. The predictive significance of the parameters of the functional state of the endothelium and regulation of angiogenesis in the first trimester of gestation in development of placental insufficiency and early reproductive losses. Voprosy Ginekologii, Akusherstva i Perinatologii. 2015;14(2):14-23. (In Russ).
  6. Tamrazyan AA, Makarov OV, Kerchelaeva SB, et al. Prognostic significance of antibodies to anexin-5 in the complications development of pregnancy and childbirth. RUDN Journal of Medicine. 2011;(6): 366-72. (In Russ).
  7. Degrelle SA, Gerbaud P, Leconte L, et al. Annexin-A5 organized in 2D-network at the plasmalemma eases human trophoblast fusion. Scientific Reports. 2017;7:42173. doi: 10.1038/srep42173
  8. Medvedev BI, Syundyukova EG, Sashenkov SL. Placental expression of erythropoietin in preeclampsia. Russian Bulletin of the Obstetrician-Gynecologist. 2015;(1):4-8. (In Russ). doi: 10.17116/rosakush20151514-8
  9. Milovanov AP. Patologiya sistemy mat’-platsenta-plod. Moscow: Meditsina; 1999. (In Russ).
  10. Beznoshchenko GV, Kravchenko EN, Rogova EV, et al. Placental insufficiency and the uterine placental area in pregnant women with preeclampsia. Russian Bulletin of the Obstetrician-Gynecologist. 2014; (5):4-8. (In Russ).
  11. Sidorova IS, Milovanov AP, Nikitina NA, et al. Specific features of placentation in preeclampsia and eclampsia. Russian Bulletin of the Obstetrician-Gynecologist. 2014;(3):4-10. (In Russ).
  12. Kulida LV, Rokotyanskaya EA, Malyshkina AI, et al. Placental morphological and immunohistochemical changes in preeclampsia and their relationship to perinatal outcomes. Russian Bulletin of the Obstetrician-Gynecologist. 2019;19(1):27-32. (In Russ). doi: 10.17116/rosakush20191901127
  13. Danisik H, Bogdanova N, Markoff A. Micromolar Zinc in Annexin A5 Anticoagulation as a Potential Remedy for RPRGL3-Associated Recurrent Pregnancy Loss. Reproductive Sciences. 2019;26(3): 348-56. doi: 10.1177/1933719118773497
  14. Aranda F, Udry S, Wingeyer SP, et al. Maternal carriers of the ANXA5 M2 haplotype are exposed to a greater risk for placenta-mediated pregnancy complications. Journal of Assisted Reproduction and Genetics. 2018;35(5):921-8. doi: 10.1007/s10815-018-1142-4
  15. Rand JH, Wu X-X, Quinn AS, et al. Hydroxy-chloroquine Protects the Annexin A5 Anticoagulant Shield From Disruption by Antiphospholipid Antibodies: Evidence for a Novel Effect for an Old Antimalarial Drug. Blood. 2010;115(11):2292-9. doi: 10.1182/blood-2009-04-213520
  16. Kulida LV, Rokotyanskaya EA, Panova IA. Immunogistokhimicheskiye parametry strukturnoy perestroyki v platsente pri gipertenzivnykh rasstroystvakh u beremennykh. Akusherstvo i Ginekologiya. 2019;(Suppl 4):72. (In Russ).
  17. Medvedev BI, Syundyukova EG, Sashenkov SL. Serum erythropoietin and its placental expression in preeclampsia-complicated pregnancy. Akusherstvo i Ginekologiya. 2015;(10):47-53. (In Russ).

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