Application of quantitative multiplex real-time polymerase chain reaction for identification of causative agents of urinary tract infections in pregnancy

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Urinary tract infections (UTIs) represent the most common bacterial infections and often complicate pregnancy. UTIs in pregnancy are classified by site of bacterial proliferation as follows: asymptomatic bacteriuria, cystitis, pyelonephritis. Screening for asymptomatic bacteriuria is a standard of obstetrical care and is included in most international guidelines. The urine microbiologic culture is considered the gold standard for laboratory diagnosis of UTIs. Molecular methods enable to rapidly detect, identify and quantitate causative agents of UTIs in urine and are effective alternative to traditional bacteriological methods.

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About the authors

Tatyana Alekseyevna Khusnutdinova

D. O. Ott Research Institute of Obstetrics and Gynecology RAMS

Researcher, Laboratory of Microbiology

Yuliya Anatolyevna Savochkina

Central Research Institute of Epidemiology

PhD, Senior Researcher, Laboratory of Molecular Diagnostics and Epidemiology of Reproductive Tract Infections, Department of Mo-lecular Diagnostics and Epidemiology

Aleksandr Yevgenyevich Gushchin

Central Research Institute of Epidemiology

PhD, Head of the Laboratory of Molecular Diagnostics and Epidemiology of Reproductive Tract Infections, Department of Molecular Diagnostics and Epidemiology

Yelena Vasilyevna Shipitsyna

D. O. Ott Research Institute of Obstetrics and Gynecology RAMS

PhD, Senior Researcher, Laboratory of Microbiology

Alevtina Mikhailovna Savicheva

D. O. Ott Research Institute of Obstetrics and Gynecology RAMS

MD, PhD, Dr Med Sci, Professor, Laboratory of Microbiology


  1. Никонов А. П., Асцурова О. Р., Каптильный В. А. Инфекции мочевыводящих путей и беременность //Гинекология. - 2007. - Т. 9, № 1.
  2. «Об утверждении Порядка оказания медицинской помощи по профилю «акушерство и гинекология (за исключением использования вспомогательных репродуктивных технологий)». Приказ Министерства здравоохранения РФ от 01.10.2012 г. № 572 н
  3. Шехтман М. М. Руководство по экстрагенитальной патологии у беременных. Практическое пособие. - М.: Триада-Х, 2005. - 816 с.
  4. Alwall N., Lohi A. Factors affecting the reliability of screening tests for bacteriuria // I. Acta Med. Scand. - 1973. - Vol. 193. - P. 499-503.
  5. Bachman J. W., Heise R. H., Naessens J. M. et al. A study of various tests to detect asymptomatic urinarytract infections in an obstetric population // JAMA. - 1993. - Vol. 270, N 16. - P. 1971-1974.
  6. Gilstrap L. C. 3rd, Cunningham F. G., Whalley P. J. Acute pyelonephritis in pregnancy: an anterospective study // Obstet. Gynecol. - 1981. - Vol. 57, N 4. - P. 409-413.
  7. Gilstrap L. C., Ramin S. M. Urinary tract infections during pregnancy // Obstet. Gynecol. Clin. North Am. - 2001. - Vol. 28, N3. - P. 581-91.
  8. Guidelines on the management of urinary and male genital tract infections. Grabe M. (chairman), Bishop M. C., Bjerklund-Johansen T. E. et al. // European Association of Urology. - 2011.
  9. Harris R. E., Gilstrap L. C. 3rd. Cystitis during pregnancy: a distinct clinical entity // Obstet. Gynecol. - 1981. - Vol. 57, N 5. - P. 578-80.
  10. Jeyabalan A., Lain K. Y. Anatomic and functional changes of the upper urinary tract during pregnancy // Urol. Clin. North Am. - 2007. - Vol. 34, N 1. - P. 1-6.
  11. Kass E. H. Bacteriuria and the diagnosis of infections of the urinary tract // Arch. Intern. Med. - 1957. - Vol. 100, N 5. - P. 709-714.
  12. Le J., Briggs F. F., McKeown A. et al. Urinary tract infections during pregnancy // Ann. Pharmacother. - 2004. - Vol. 38, N 10. - P. 1692-701.
  13. Lindsay E. N. Managing recurrent urinary tract infections in women // Women’s Health. - 2005. - Vol. 1, N 1. - P. 39-50.
  14. Millar L. K., Cox S. M. Urinary tract infections complicating pregnancy // Infect Dis. Clin. North Am. - 1997. - Vol. 11, N 1. - P. 13-26.
  15. National Collaborating Centre for Women's and Children's Health. Clinical Guidelines. Antenatal Care. Routine care for the healthy pregnant woman. London: National Institute for Clinical Excellence, 2003.
  16. Nicolle L. E. Screening for asymptomatic bacteriuria in pregnancy // In: Canadian Guide to Clinical and Preventative Health Care. Ottawa: Health Canada, 1994. - P. 100-106.
  17. Nicolle L. E., Bradley S., Colgan R. et al. Infectious Diseases Society of America guidelines for the diagnosis and treatment of asymptomatic bacteriuria in adults // Clin. Infect. Dis. - 2005. - Vol.40. - P. 643-654.
  18. Pfaller M. A., Koontz F. P. Laboratory evaluation of leukocyte esterase and nitrite tests for the detectionof bacteriuria // J. Clin. Microbiol. - 1985. - Vol. 21, N 5. - P. 840-842.
  19. Romero R., Oyarzun E., Mazor M. et al. Meta-analysis of the relationship between asymptomatic bacteriuria and preterm delivery / low birth weight // Obstet. Gynecol. - 1989. - Vol. 73, N 4. - P. 576-582.
  20. Sharma P., Thapa L. Acute pyelonephritis in pregnancy: a retrospective study // Aust. N Z J. Obstet. Gynaecol. - 2007. - Vol. 47, N 4. - P. 313-315.
  21. Wing D. A. Pyelonephritis // Clin. Obstet. Gynecol. - 1998. - Vol. 41, N 3. - P. 515-526.



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Copyright (c) 2014 Khusnutdinova T.A., Savochkina Y.A., Gushchin A.Y., Shipitsyna Y.V., Savicheva A.M.

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