Antimicrobial activity of black poplar (Populus migra L.) bud and sprout tinctures

Introduction. Searches for drugs are currently underway to treat cystic fibrosis, a disease that is caused by a gene mutation and is characterized by impaired secretion by exocrine glands in vital organs. Patients infected with Burkholderia strains that are resistant to many currently available antibacterial drugs present the greatest problem for the therapy of cystic fibrosis. The promising source of biologically active compounds is black poplar (Populus nigra L.), the buds of which have a pronounced antibacterial effect. Objective: to comparatively investigate the antibacterial activity of black poplar bud and sprout tinctures in ethanol at various concentrations against the strains of cystic fibrosis. Material and methods. The objects of the investigation were tinctures (1:5) of black poplar buds and sprouts in 40, 70, 80, and 96% ethanol. The minimal inhibitory concentration (MIC) was estimated using two-fold serial dilutions in a broth medium. Clinical strains, such as Burkholderia cenocepacia ST, Burkholderia multivorans, Pseudomonasaeruginosa, from patients with cystic fibrosis served as test cultures for determining the antimicrobial activity of the tinctures. The comparison objects were ethanol at various concentrations and working standard sample solution of pinostrobin isolated from black poplar buds. Results. Poplar sprout tinctures were found to have a wide spectrum of bacteriostatic activity against all the strains tested. The samples studied had marked bactericidal and bacteriostatic activities. MIC against Burkholderia multivorans was found in the poplar bud tincture in 80% ethanol. All the tinctures tested showed a low antimicrobial activity against Pseudomonas aeruginosa. Conclusion. The results of the investigation can serve as a rationale for designing new drugs based on black poplar buds and sprouts as an agent for the treatment of cystic fibrosis.

Populus nigra L, cystic fibrosis, black poplar, Populus nigra L, poplar buds, poplar sprouts, tinctures, antibacterial activity