STUDY OF THE FORMATION OF CANDIDA ALBICANS AND ESCHERICHIA COLI BIOFILMS

Introduction. Biofilms are a form of microbial communities fixed onto various abiotic and biotic surfaces. This way in which the microorganisms exist creates major problems in pharmaceutical, medical, and veterinary practice. The creation of biofilms by microorganisms makes them inaccessible to the vast majority of antimicrobial agents. The existence of biofilms in chronic infections requires entirely new treatment approaches. Some mechanisms for the formation and maintenance of certain types of biofilms are not yet completely clear. Objective: to comparatively assess and select effective methods for studying the structural and functional features of the formation of biofilms by microscopic fungi and bacteria for the further design of effective pharmacological preparations. Results. The investigators studied the structural and functional features of non-pathogenic strains of microscopic fungi of the genus Candida and bacteria Escherichia coli on the ability to form biofilms by a new modified method. A simple and economical method for producing biofilms was developed, which allows one to study the role of the tube formation process in Candida-Escherichia communities. Conclusion. The proposed model can be used to study the therapeutic effect of various drugs on biofilms and the mechanisms of their initial adhesion.

microscopic fungi, bacteria, biofilms, adhesive properties, matrix, microscopy